LOCUS       sequence-152513-         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB135
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(99..105,106..123,124..129))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(166..290)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
       61 tcctgaaaat ctcgacggat cctaactcaa aatccacaca ttatacgagc cggaagcata
      121 aagtgtaaag cctggggtgc ctaatgcggc ccctcgaggg gccgccatag tgactggata
      181 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
      241 atatttatat cattttacgt ttctcgttca gcttttttgt acaaacttgt tgataactct
      301 agcttgtgcc ccagtttgct agggaggtcg cagtatctgg ccacagccac ctcgtgctgc
      361 tcgacgtagg tctctttgtc ggcctccttg attctttcca gtctgtggtc cacatagtag
      421 acgccgggca tcttgaggtt cttagcgggt ttcttggatc tgtatgtggt cttgaagttg
      481 cagatcaggt ggcccccgcc cacgagcttc agggccatgt cgcttctgcc ttccaggccg
      541 ccgtcagcgg ggtacagcat ctcggtgttg gcctcccagc cgagtgtttt cttctgcatc
      601 acagggccgt tggatgggaa gttcacccct ctgatcttga cgttgtagat gaggcagccg
      661 tcctggaggc tggtgtcctg ggtagcggtc agcacgcccc cgtcttcgta tgtggtgact
      721 ctctcccatg tgaagccctc ag
//
