LOCUS       sequence-152517-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB221
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(91..97,98..115,116..121))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(158..282)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
       61 atctcgacgg atcctaactc aaaatccaca cattatacga gccggaagca taaagtgtaa
      121 agcctggggt gcctaatgcg gcccctcgag gggccgccat agtgactgga tatgttgtgt
      181 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
      241 atcattttac gtttctcgtt cagctttttt gtacaaactt gttgataact ctagtcaacc
      301 tttctgcagg cacatcaata ctgttgaggc aacacccata gaagcacgga accaaggagc
      361 accgacgtcc atagcacaca gccaacctcg ctgaccttcg actcctcttc aagtcaagga
      421 tagacaaatc tatcatgcag gaaacttacg aggcacctcc gcaccagaga ccgaactgaa
      481 caccttaccg agataacagg actgaccaaa gtgggatgta cttggctaaa acgaacctaa
      541 aaaatcactc gctcgaggcc ccaatcgaca ccacgtaaaa gctccagtcg ggtttcaggt
      601 ctagacaatc acaatatcga gcaatcgtaa cgacgtacag agacgagggc acaaggcgca
      661 taccagaaga caagatgcag ctcttcgatg tcgatgctct ccacgcaaga gagaaagaag
      721 tgaaaggaac tctagggttg cggcgatcga ttttaagcca gacgctccac tgcctcccag
      781 cgagctttga gagcaagcgc gaccacagca ctagacgcc
//
