LOCUS       sequence-152520-         690 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB222
FEATURES             Location/Qualifiers
     source          1..690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(89..95,96..113,114..119))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(156..280)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(302..647)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(339..359)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
       61 ctcgacggat cctaactcaa aatccacaca ttatacgagc cggaagcata aagtgtaaag
      121 cctggggtgc ctaatgcggc ccctcgaggg gccgccatag tgactggata tgttgtgttt
      181 tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat
      241 cattttacgt ttctcgttca gcttttttgt acaaacttgt tgataactct agagtccccc
      301 gtgttctctc caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt
      361 gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag
      421 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
      481 gaccactgtc ggcagaggca tcttcaacga tggcctttcc tttatcgcaa tgatggcatt
      541 tgtaggagcc accttccttt tccactatct tcacaataaa gtgacagata gctgggcaat
      601 ggaatccgag gaggtttccg gatattaccc tttgttgaaa agtctcaatt gccctttggt
      661 cttctgagac tgtatctttg atatttttgg
//
