LOCUS       sequence-152523-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MCS-containing binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB300
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    16..37
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    122..296
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 agcctggggt gcctaatgag tgagctaact cacattaggc tgaattaggc gcgcctattt
       61 ctgaattcaa cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg
      121 ggatctggat tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt
      181 attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc
      241 ctataggaac cctaattccc ttatctggga actactcaca cattattatg gagaaactcg
      301 agttagtata tagtccttcc atcaccctct gtgataatct ccttaaaact tgctccccca
      361 ggaatcatag gcagaacgtg ctcctggtgg ggaactatca catccagtaa ataaggaccg
      421 ggagaatcta gcatagtttg aattgctgct cttagttcat ccttcttcac cacacgcgca
      481 tttggcacct tgaaaccgtc tgcaactgta cagaagtcgg ggtagatctg ctcctcgtga
      541 tctggatcac ctaagtaagt atgtgctctg ttggccttgt agaatctatc ctccaattgt
      601 acgaccattc ctagatgttg gttgtttaag atcatgatct tcacgggtaa cttctccaca
      661 aaaattgtag ccaactcctg tacgttcatc acaaagctac catcgccatc gatgtcgatc
      721 acaggaatat ctggttgagt tgcggcagcc cccaaagcag aaggaagtcc gaatcc
//
