LOCUS       sequence-152533-         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB310
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(105..229)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             240..305
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      324..576
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(609..625)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(609..625)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(622..644)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    633..649
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(657..663,664..681,682..687))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat
       61 ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt gactggatat
      121 gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga
      181 tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt gataacagcg
      241 actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag gatgacgatg
      301 acaagtagag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt ttcttaagat
      361 tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc
      421 atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag
      481 tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata
      541 aattatcgcg cgcggtgtca tctatgttac tagatcggga attggttccg gaaccaattc
      601 gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      661 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
//
