LOCUS       sequence-152548-        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Coseno M, Martin G, Berger C, Gilmartin G, Keller W, Doublie S
  TITLE     Crystal structure of the 25 kDa subunit of human cleavage factor Im.
  JOURNAL   Nucleic Acids Res. 2008 Jun;36(10):3474-83. doi: 10.1093/nar/gkn079.
            Epub 2008 Apr 29.
  PUBMED    18445629
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkn079"; journalName: "Nucleic Acids Res"; date: 
            "2008-06"; volume: "36"; issue: "10"; pages: "3474-83"
COMMENT     Sequence Label: HisMBP CFlm25
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..34
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(721..745)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(757..774)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(757..773)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(766..788)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(952..969)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 aaaagcaggc tcggagaacc tgtacttcca gggtatgtct gtggtaccgc ccaatcgctc
       61 gcagaccggc tggccccggg gggtcactca gttcggcaac aagtacatcc agcagacgaa
      121 gcccctcacc ctggagcgca ccatcaacct gtaccctctt accaattata cttttggtac
      181 aaaagagccc ctctacgaga aggacagctc tgttgcagcc agatttcagc gcatgaggga
      241 agaatttgat aaaattggaa tgaggaggac tgtagaaggg gttctgattg tacatgagca
      301 ccggctaccc catgtgttac tgctgcagct gggaacaact ttcttcaaac tacctggtgg
      361 tgaacttaac ccaggagaag atgaagttga aggactaaaa cgcttaatga cagagatact
      421 gggtcgtcag gatggagttt tgcaagactg ggtcattgac gattgcattg gtaactggtg
      481 gagaccaaat tttgaacctc ctcagtatcc atatattcct gcacatatta caaagcctaa
      541 ggaacataag aagttgtttc tggttcagct tcaagaaaaa gccttgtttg cagtccctaa
      601 aaattacaag ctggtagctg caccattgtt tgaattgtat gacaatgcac caggatatgg
      661 acccatcatt tctagtctcc ctcagctgtt gagcaggttc aattttattt acaactaata
      721 acccagcttt cttgtacaaa gtggtgatag cttggcactg gccgtcgttt tacaacgtcg
      781 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
      841 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
      901 gaatggcgaa tggcagcttg gctgttttgg cggatgagat aagattttca gcctgataca
      961 gattaaatca gaacgcagaa gcgggtctga taaaacagaa tttgcctggc g
//
