LOCUS       sequence-152549-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Coseno M, Martin G, Berger C, Gilmartin G, Keller W, Doublie S
  TITLE     Crystal structure of the 25 kDa subunit of human cleavage factor Im.
  JOURNAL   Nucleic Acids Res. 2008 Jun;36(10):3474-83. doi: 10.1093/nar/gkn079.
            Epub 2008 Apr 29.
  PUBMED    18445629
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkn079"; journalName: "Nucleic Acids Res"; date: 
            "2008-06"; volume: "36"; issue: "10"; pages: "3474-83"
COMMENT     Sequence Label: HisMBP CFlm25
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(697..717)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(720..744)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(757..780)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     regulatory      888..897
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gaagctgggt tattagttgt aaataaaatt gaacctgctc aacagctgag ggagactaga
       61 aatgatgggt ccatatcctg gtgcattgtc atacaattca aacaatggtg cagctaccag
      121 cttgtaattt ttagggactg caaacaaggc tttttcttga agctgaacca gaaacaactt
      181 cttatgttcc ttaggctttg taatatgtgc aggaatatat ggatactgag gaggttcaaa
      241 atttggtctc caccagttac caatgcaatc gtcaatgacc cagtcttgca aaactccatc
      301 ctgacgaccc agtatctctg tcattaagcg ttttagtcct tcaacttcat cttctcctgg
      361 gttaagttca ccaccaggta gtttgaagaa agttgttccc agctgcagca gtaacacatg
      421 gggtagccgg tgctcatgta caatcagaac cccttctaca gtcctcctca ttccaatttt
      481 atcaaattct tccctcatgc gctgaaatct ggctgcaaca gagctgtcct tctcgtagag
      541 gggctctttt gtaccaaaag tataattggt aagagggtac aggttgatgg tgcgctccag
      601 ggtgaggggc ttcgtctgct ggatgtactt gttgccgaac tgagtgaccc cccggggcca
      661 gccggtctgc gagcgattgg gcggtaccac agacataccc tggaagtaca ggttctccga
      721 gcctgctttt ttgtacaaac ttgtgatcga attagtctgc gcgtctttca gggcttcatc
      781 gacagtctga cgaccgctgg cggcgttgat caccgcagta cgcacggcat accagaaagc
      841 ggacatctgc gggatgttcg gcatgatttc acctttctgg gcgttttcca tggtggcggc
      901 aatacgtgga tctttcgcca actcttcctc gtaagacttc agcgctacgg
//
