LOCUS       sequence-152555-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB407
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(99..105,106..123,124..129))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(166..290)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(316..333)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(316..332)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(325..347)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    535..559
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
       61 tcctgaaaat ctcgacggat cctaactcaa aatccacaca ttatacgagc cggaagcata
      121 aagtgtaaag cctggggtgc ctaatgcggc ccctcgaggg gccgccatag tgactggata
      181 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
      241 atatttatat cattttacgt ttctcgttca gcttttttgt acaaacttgt tgataactct
      301 agacccaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
      361 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
      421 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag
      481 cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag
      541 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa
      601 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
      661 ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg
      721 acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca
      781 ggctgccgcc ctgccctttt cctggcgttt
//
