LOCUS       sequence-152556-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing his-MBP tagged AsCpf1 (E.coli codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Hur JK, Kim K, Been KW, Baek G, Ye S, Hur JW, Ryu SM, Lee YS, Kim JS
  TITLE     Targeted mutagenesis in mice by electroporation of Cpf1 
            ribonucleoproteins.
  JOURNAL   Nat Biotechnol. 2016 Jun 6. doi: 10.1038/nbt.3596.
  PUBMED    27272385
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jun 6. doi: 10.1038/nbt.3596."
COMMENT     Sequence Label: pDEST-hisMBP-AsCpf1-EC
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..41)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(15..41)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(48..68)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gaagctgggt gttaggcgta gtcgggcacg tcgtaggggt aaccacctac ttttctcttt
       61 ttctttgggt ttctcagttc ttgaatgtag gccagccagt cctgattcga gatgccgttc
      121 tgcagtttca gatcttttga ttctttcaaa tggttcagta ggagctgacc tttgagagcg
      181 atatggtaag ctccattggc atcggcatcc atcggccact ccggattttg aaatctgcta
      241 tcgaaacaaa cgccgttcag atcacggacc gggctattaa tgtagtcttc ccccgtagcc
      301 gcattactgt tacgcatctg cagcacgctt cggatcagcg ctaccatggt gtctattgcg
      361 tggctgtcat cgttctccag cagcttcggc aggatgtttg atccatcccg aaagacaata
      421 cctttctcct ccagcagcgc tatcaattca tttgccgggt acaagtccct ataccgtccg
      481 gtgaagcggt gattttctat aacgggcaca atgcgtttcc ccgcaataaa gggggtacct
      541 ttggcatcaa actgtgtctc attcttttca aaaacaatgt cccacgccgg cataaagcca
      601 ggcaggccgc gctgaaagga cagattacgg ttcattttaa aatgcagaat gaagtcgccg
      661 gttttcacgt catagtgcag aaaatcaaag ccctccagaa aatgtttgcg actttcatga
      721 tttttaatgg ttttccagac aaacggatct acgaaacctg tcagcgggtc tatcttgctg
      781 gtatatggcg ccggaacata gaacaggaaa ccgctctgcg ttcccatttt tgcaaagctc
      841 gtaaattgat cagtcagctg gtacgggttc agcacaccgc ccaccttctc tgccggataa
      901 tctttcagaa ccaggcaatt cagcttgtct atcagcattt tctcgaactg ctgataaacg
      961 gctttttcag cgatccc
//
