LOCUS       sequence-152557-         650 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB407
FEATURES             Location/Qualifiers
     source          1..650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(102..226)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(282..303)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(543..562)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata
       61 aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt
      121 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
      181 ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggttgat aacagcatgg
      241 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
      301 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
      361 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
      421 tgaccacctt cggctacggc ctgatgtgct tcgcccgcta ccccgaccac atgaagcagc
      481 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
      541 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
      601 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
//
