LOCUS       sequence-152558-        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing his-MBP tagged AsCpf1 (E.coli codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Hur JK, Kim K, Been KW, Baek G, Ye S, Hur JW, Ryu SM, Lee YS, Kim JS
  TITLE     Targeted mutagenesis in mice by electroporation of Cpf1 
            ribonucleoproteins.
  JOURNAL   Nat Biotechnol. 2016 Jun 6. doi: 10.1038/nbt.3596.
  PUBMED    27272385
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jun 6. doi: 10.1038/nbt.3596."
COMMENT     Sequence Label: pDEST-hisMBP-AsCpf1-EC
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..46
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aaaagcaggc ttcatgggtg ctcctccaaa aaagaagaga aaggtaggtg gtatgacaca
       61 gtttgaaggc ttcaccaatc tctaccaggt cagcaagacg ctacgttttg agcttatccc
      121 gcagggaaaa accctgaaac acattcagga acaggggttc atagaggaag ataaggcgcg
      181 taacgaccat tataaagaac tgaagcctat aatcgaccgt atttataaaa cgtacgcgga
      241 tcagtgcctg cagctggttc agctggattg ggagaatctg tccgcggcta ttgatagcta
      301 tcgcaaagag aagaccgagg aaacccgtaa cgcactgatt gaagagcagg cgacctatcg
      361 gaatgcgatc catgattact tcatcggccg caccgacaac ctgaccgatg caattaacaa
      421 acgtcacgca gagatttaca aaggtctgtt taaagcagag ttattcaatg gcaaggttct
      481 gaaacagctg ggtacggtca ccaccaccga acacgaaaac gcactgctga ggagctttga
      541 taaatttacc acatatttca gcggtttcta tgaaaatcgt aagaatgtat ttagcgccga
      601 agatatttcc accgcaattc ctcatcgtat tgtgcaggat aattttccga agtttaaaga
      661 aaattgtcat atttttaccc gtctgatcac cgcggtaccg agcctgcgag agcattttga
      721 aaacgttaag aaagccattg gaatttttgt cagtaccagc attgaagaag tgttttcgtt
      781 cccgttctat aaccaactgc tgacccagac ccagattgat ctgtacaatc agctgctggg
      841 gggcataagc cgcgaggcag gtaccgaaaa gataaaggga ctcaatgagg tgctgaatct
      901 ggcaattcag aagaatgatg aaacggctca tatcattgct agcctgccgc atcgtttcat
      961 tcccctgttt aagcaaatcc tgagcgatcg caatacactg agctttatcc tcgaagagtt
     1021 taaatcggac gaagaa
//
