LOCUS       sequence-152565-       13696 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13696)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 13696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB335
FEATURES             Location/Qualifiers
     source          1..13696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        794..1139
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1150..1839
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGH"
     protein_bind    1855..1979
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2016..2021,2022..2039,2040..2046)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2100..2759
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2166..2185)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3101..3406
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3285..3304
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3447..3571)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3603..3855
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3889..3905)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3889..3905)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3902..3924)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3913..3929
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3937..3943,3944..3961,3962..3967))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3982..4003
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4088..4263
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(6347..7023)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    7218..7242
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7763..8554
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8800..9388
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9289..9308
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9542..9559
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9574..9714
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9640..9659)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9800..9822
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10058..10252
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10318..11391)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11820..12449)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttagat tagccttttc aatttcagaa agaatgctaa cccacagatg gttagagagg
      361 cttacgcagc aggtctcatc aagacgatct acccgagcaa taatctccag gaaatcaaat
      421 accttcccaa gaaggttaaa gatgcagtca aaagattcag gactaactgc atcaagaaca
      481 cagagaaaga tatatttctc aagatcagaa gtactattcc agtatggacg attcaaggct
      541 tgcttcacaa accaaggcaa gtaatagaga ttggagtctc taaaaaggta gttcccactg
      601 aatcaaaggc catggagtca aagattcaaa tagaggacct aacagaactc gccgtaaaga
      661 ctggcgaaca gttcatacag agtctcttac gactcaatga caagaagaaa atcttcgtca
      721 acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag
      781 accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc
      841 attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca
      901 aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc
      961 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt
     1021 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
     1081 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagagaacac
     1141 gagcttacaa tgagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc
     1201 accgtgaaca accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc
     1261 acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc
     1321 ctggctacca gcttcatgta cggcagcaga accttcatca accacaccca gggcatcccc
     1381 gacttcttta agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa
     1441 gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac
     1501 aacgtcaaga tcagaggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca
     1561 ctcggctggg aggccaacac cgagatgctg taccccgctg acggcggcct ggaaggcaga
     1621 agcgacatgg ccctgaagct cgtgggcggg ggccacctga tctgcaactt caagaccaca
     1681 tacagatcca agaaacccgc taagaacctc aagatgcccg gcgtctacta tgtggaccac
     1741 agactggaaa gaatcaagga ggccgacaaa gagacctacg tcgagcagca cgaggtggct
     1801 gtggccagat actgcgacct ccctagcaaa ctggggcaca agctagagtt atcaacaagt
     1861 ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag
     1921 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg
     1981 cggcccctcg aggggccgca ttaggcaccc caggctttac actttatgct tccggctcgt
     2041 ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa
     2101 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     2161 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     2221 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     2281 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
     2341 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     2401 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     2461 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     2521 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     2581 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     2641 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     2701 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa
     2761 gatctggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt
     2821 gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat
     2881 gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca aggcatatat
     2941 gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt
     3001 gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa
     3061 atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac
     3121 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
     3181 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc
     3241 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
     3301 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
     3361 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct
     3421 tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt
     3481 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
     3541 tttctcgttc agctttcttg tacaaagtgg ttgataacag cgcttagagc tcgaatttcc
     3601 ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
     3661 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
     3721 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
     3781 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     3841 ctatgttact agatcgggaa ttggttccgg aaccaattcg taatcatggt catagctgtt
     3901 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     3961 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaggctgaa ttaggcgcgc
     4021 ctatttctga attcaacaca ttgcggacgt ttttaatgta ctgaattaac gccgaattaa
     4081 ttcgggggat ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat
     4141 agaagtattt tacaaataca aatacatact aagggtttct tatatgctca acacatgagc
     4201 gaaaccctat aggaacccta aattccctta tctgggaact actcacacat tattatggag
     4261 aaactcgaag ttagtatata gtccttccat caccctctgt gataatctcc ttaaaacttg
     4321 ctcccccagg aatcataggc agaacgtgct cctggtgggg aactatcaca tccagtaaat
     4381 aaggaccggg agaatctagc atagtttgaa ttgctgctct tagttcatcc ttcttcacca
     4441 cacgcgcatt tggcaccttg aaaccgtctg caactgtaca gaagtcgggg tagatctgct
     4501 cctcgtgatc tggatcacct aagtaagtat gtgctctgtt ggccttgtag aatctatcct
     4561 ccaattgtac gaccattcct agatgttggt tgtttaagat catgatcttc acgggtaact
     4621 tctccacaaa aattgtagcc aactcctgta cgttcatcac aaagctacca tcgccatcga
     4681 tgtcgatcac aggaatatct ggttgagttg cggcagcccc caaagcagaa ggaagtccga
     4741 atcccatggc tccaagacct ccagatgtca accaacgccg cggttcgttg tacttgaacc
     4801 actgggctgc ccacatttga tgttggccaa caccagtaga tatgatggca tttccaccag
     4861 tgagttcgta cagcgtctgt atggcagatt gaggagcaat ggcatcctcg aatacgggaa
     4921 acttcattgg ccatttctgc ttttgttctt ctagttctgc cacccagttg gagaagtcga
     4981 aagtcctctt ttgactcttc aagagggcat tgatttcact tagcgcaggt ttcacatcag
     5041 cacacatgga cacatggggc tgtttgttct ttccaatttc agcagggtca atatcgatat
     5101 gaacgatgct ggacctgcta gcaaaagtct ccagtttacc agtcactcga tcgtcgaaac
     5161 gcactccaaa tgccaacaac agatctgctt ggtccacggc gtagttggcg taaacagttc
     5221 catgcattcc aagcattttc aaacttaatg gatcagactc gggatacgct cccaaaccca
     5281 ttaaagtcga tgtgatagga attccggtaa gctcgacaaa ttccctcaac tcttcgctgg
     5341 cgtgattgca acctcctcca gaatacacta caggcttctt agaactagaa atgagtctta
     5401 taatctgctc gattaacgtt ggttccggtg gccgaggcag tcgttgtaag tagcccttca
     5461 gttgcattgg ctgagaccaa tctggaatag ccatttgctg ttgcacgtcc ttgggaatat
     5521 ctaccaagac tgggcccggc ctacctgaag ctgccaaaaa gaaagcctcc ctgattacgc
     5581 gagggatgtc atcaatgttc ataacaaggt aattgtgctt ggtgatcgat ctcgtaacct
     5641 ccacaattgg agtttcttga aaagcatcgg taccaatcat cctactggat acttgacctg
     5701 taatggccac tagtggcaca ctgtccaaga gggcatcagc gagcccggta acaagattgg
     5761 tcgctccagg accggatgtg gctatacaaa cccctactcg tccagacgat ttagcataac
     5821 cttcagcagc gaaaatctcc ccctgctcgt ggcgacagag aatgttgcgg atgctgctgg
     5881 aacgtgtaag agcctggtga atctccatag acgctccgcc gggataagcg aacaccgttc
     5941 taatcccttc tcgctcaaga gcctccacca aaatgtcggc tcctttgcgg ggctcgtcat
     6001 ccttgtagcg agactgaaag tcggacgggg ttggtgcaac gagtgacttc ccgctgctaa
     6061 gccgtggtcc actcacatcg actgcagact ggatcttctc gctaacagct tgggctttca
     6121 cttccagtga acgctcagtc ttccgagagg tgttgtatgc atgtcccctc agagcggctc
     6181 ccgagaagaa accactcctc acctttagct ctttagctcc cttgcaacaa gaccttgccg
     6241 atggctgcac cttctccagg ctcgctatgc tcaaaggcac agctgcgcat ccactaattg
     6301 acgccatgct cgagagagat agatttgtag agagagactg gtgatttcag cgtgtcctct
     6361 ccaaatgaaa tgaacttcct tatatagagg aagggtcttg cgaaggatag tgggattgtg
     6421 cgtcatccct tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg
     6481 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
     6541 cggcagaggc atcttgaacg atagcctttc ctttatcgca attatggcat ttgtaggtgc
     6601 caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga
     6661 ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga
     6721 ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttcacatcaa
     6781 tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt
     6841 gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta
     6901 tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga
     6961 cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc
     7021 tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg
     7081 tcgtgctcca ccatgttggc aagctgctct agccaatacg caaaccgaat tcagaaataa
     7141 attcagccta attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt
     7201 tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc
     7261 cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac
     7321 ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc
     7381 ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat
     7441 gttgattgta acgatgacag agcgttgctg cctgtgatca attcgggcac gaacccagtg
     7501 gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg
     7561 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     7621 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     7681 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     7741 ccctaaaaca aagttaaaca tcatggggga agcggtgatc gccgaagtat cgactcaact
     7801 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     7861 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
     7921 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     7981 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     8041 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     8101 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     8161 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     8221 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     8281 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     8341 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     8401 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     8461 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
     8521 gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc
     8581 gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg cactaagcac ataattgctc
     8641 acagccaaac tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct
     8701 acacaaattg ggagatatat catgcatgac caaaatccct taacgtgagt tttcgttcca
     8761 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     8821 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     8881 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     8941 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     9001 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     9061 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     9121 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     9181 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     9241 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     9301 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     9361 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     9421 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     9481 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     9541 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     9601 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     9661 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     9721 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     9781 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     9841 aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc
     9901 ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc ggccagcggc
     9961 cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc
    10021 tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca ggcgggtttt
    10081 aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt tctcttttat
    10141 atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc caatgtacgg
    10201 gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca caggaaagag
    10261 accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct ccgtacatta
    10321 ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta ggatcgggcc
    10381 agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc cgatcagctt
    10441 gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt
    10501 cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa
    10561 acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca cgtccgggtt
    10621 cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga tgtactccgg
    10681 ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac cctcggatac
    10741 cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt
    10801 taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca
    10861 gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc
    10921 ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta ccaggtcgta
    10981 atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc cgtctggaag
    11041 ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga aaacggccac
    11101 gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc
    11161 tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg ccggcggttg
    11221 ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc
    11281 tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca ccaggtcatc
    11341 acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca cgccgattcc
    11401 tcgggcttgg gggttccagt gccattgcag ggccggcaga caacccagcc gcttacgcct
    11461 ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc tggttgttct
    11521 tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta ttcatttgct
    11581 catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg tcttgccttg
    11641 gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa agttgacccg
    11701 cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc tgcgtgcgct
    11761 cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac ctcattaact
    11821 caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc
    11881 tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc
    11941 tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca
    12001 ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg tagccttcca
    12061 tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc ccatatgtcg
    12121 taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc ggacacagcc
    12181 aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc gatggccttc
    12241 acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc
    12301 acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag aacatcggcc
    12361 ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct
    12421 ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt ttatttactc
    12481 atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat acacatcacc
    12541 tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag gccgccagct
    12601 tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg tgcgcgggcg
    12661 gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg
    12721 gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt cattctcggc
    12781 ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc
    12841 ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac
    12901 gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca gctcgcgggc
    12961 gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc ctcgggcctt
    13021 ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga actcggcaat
    13081 gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc
    13141 tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc
    13201 gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag
    13261 gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga
    13321 aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc
    13381 gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt tcatggcgta
    13441 atgtctccgg ttctagtcgc aagtattcta ctttatgcga ctaaaacacg cgacaagaaa
    13501 acgccaggaa aagggcaggg cggcagcctg tcgcgtaact taggacttgt gcgacatgtc
    13561 gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc agcggagggg
    13621 ttggatcaaa gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg
    13681 acgaacggat aaacct
//
