LOCUS       sequence-152567-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacillus subtilis sgRNA expression vector; integrates into amyE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Peters JM, Colavin A, Shi H, Czarny TL, Larson MH, Wong S, Hawkins 
            JS, Lu CH, Koo BM, Marta E, Shiver AL, Whitehead EH, Weissman JS, 
            Brown ED, Qi LS, Huang KC, Gross CA
  TITLE     A Comprehensive, CRISPR-based Functional Analysis of Essential Genes
            in Bacteria.
  JOURNAL   Cell. 2016 Jun 2;165(6):1493-506. doi: 10.1016/j.cell.2016.05.003. 
            Epub 2016 May 26.
  PUBMED    27238023
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.05.003"; journalName: "Cell"; date: "2016-06-2-
            2"; volume: "165"; issue: "6"; pages: "1493-506"
COMMENT     Sequence Label: pJMP2
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        741..816
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             837..866
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             882..899
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(955..972)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 aaattctcca gtcttcacat cggtttgaaa ggaggaagcg gaagaatgaa gtaagaggga
       61 tttttgactc cgaagtaagt cttcaaaaaa tcaaataagg agtgtcaaga atgtttgcaa
      121 aacgattcaa aacctcttta ctgccgttat tcgctggatt tttattgctg tttcatttgg
      181 ttctggcagg accggcggct gcgagtgctg aaacggcgaa caaatcgaat gagcttacag
      241 caccgtcgat caaaagcgga accattcttc atgcatggaa ttggtcgttc aatacgttaa
      301 aacacaatat gaaggatatt catgatgcag gatatacagc cattcagaca tctccgatta
      361 accaagtaaa ggaagggaat caaggagata aaagcatgtc gaactggtac tggctgtatc
      421 agccgacatc gtatcaaatt ggcaaccgtt acttaggtac tgaacaagaa tttaaagaaa
      481 tgtgtgcagc cgctgaagaa tatggcataa aggtcattgt tgacgcggtc atcaatcata
      541 ccaccagtga ttatgccgcg atttccaatg aggttaagag tattccaaac tggacacatg
      601 gaaacacaca aattaaaaac tggtctgatc ggatcctaga agcttatcga attccttatt
      661 aacgttgata taatttaaat tttatttgac aaaaatgggc tcgtgttgta caataaatgt
      721 aactttcagt ttagcggtct gttttagagc tagaaatagc aagttaaaat aaggctagtc
      781 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttgaagctt gggcccgaac
      841 aaaaactcat ctcagaagag gatctgaata gcgccgtcga ccatcatcat catcatcatt
      901 gagtttaaac ggtctccagc ttggctgttt tggcggatga gagaagattt tcagcctgat
      961 acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggca
//
