LOCUS       sequence-152568-         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacillus subtilis sgRNA expression vector; integrates into amyE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Peters JM, Colavin A, Shi H, Czarny TL, Larson MH, Wong S, Hawkins 
            JS, Lu CH, Koo BM, Marta E, Shiver AL, Whitehead EH, Weissman JS, 
            Brown ED, Qi LS, Huang KC, Gross CA
  TITLE     A Comprehensive, CRISPR-based Functional Analysis of Essential Genes
            in Bacteria.
  JOURNAL   Cell. 2016 Jun 2;165(6):1493-506. doi: 10.1016/j.cell.2016.05.003. 
            Epub 2016 May 26.
  PUBMED    27238023
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.05.003"; journalName: "Cell"; date: "2016-06-2-
            2"; volume: "165"; issue: "6"; pages: "1493-506"
COMMENT     Sequence Label: pJMP2
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(791..809)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttaacttgct atttctagct ctaaaacaga ccgctaaact gaaagttaca tttattgtac
       61 aacacgagcc catttttgtc aaataaaatt taaattatat caacgttaat aaggaattcg
      121 ataagcttct aggatccgat cagaccagtt tttaatttgt gtgtttccat gtgtccagtt
      181 tggaatactc ttaacctcat tggaaatcgc ggcataatca ctggtggtat gattgatgac
      241 cgcgtcaaca atgaccttta tgccatattc ttcagcggct gcacacattt ctttaaattc
      301 ttgttcagta cctaagtaac ggttgccaat ttgatacgat gtcggctgat acagccagta
      361 ccagttcgac atgcttttat ctccttgatt cccttccttt acttggttaa tcggagatgt
      421 ctgaatggct gtatatcctg catcatgaat atccttcata ttgtgtttta acgtattgaa
      481 cgaccaattc catgcatgaa gaatggttcc gcttttgatc gacggtgctg taagctcatt
      541 cgatttgttc gccgtttcag cactcgcagc cgccggtcct gccagaacca aatgaaacag
      601 caataaaaat ccagcgaata acggcagtaa agaggttttg aatcgttttg caaacattct
      661 tgacactcct tatttgattt tttgaagact tacttcggag tcaaaaatcc ctcttacttc
      721 attcttccgc ttcctccttt caaaccgatg tgaagactgg agaattttgt taattcttga
      781 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
      841 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      901 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttc
//
