LOCUS       sequence-152572-         467 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB302
FEATURES             Location/Qualifiers
     source          1..467
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(68..74,75..92,93..98))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(135..259)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(283..329)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(318..338)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 atttttttct ccattttagc ttccttagct cctgaaaatc tcgacggatc ctaactcaaa
       61 atccacacat tatacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgcggcc
      121 cctcgagggg ccgccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
      181 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag
      241 cttttttgta caaacttgtt gataactcta gagtcccccg tgttctctcc aaatgaaatg
      301 aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta
      361 cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt
      421 tttccacgat gctcctcgtg ggtgggggtc catctttggg accactg
//
