LOCUS       sequence-152574-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacillus subtilis dCas9 expression vector; integrates into 
            lacA/ganA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Peters JM, Colavin A, Shi H, Czarny TL, Larson MH, Wong S, Hawkins 
            JS, Lu CH, Koo BM, Marta E, Shiver AL, Whitehead EH, Weissman JS, 
            Brown ED, Qi LS, Huang KC, Gross CA
  TITLE     A Comprehensive, CRISPR-based Functional Analysis of Essential Genes
            in Bacteria.
  JOURNAL   Cell. 2016 Jun 2;165(6):1493-506. doi: 10.1016/j.cell.2016.05.003. 
            Epub 2016 May 26.
  PUBMED    27238023
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.05.003"; journalName: "Cell"; date: "2016-06-2-
            2"; volume: "165"; issue: "6"; pages: "1493-506"
COMMENT     Sequence Label: pJMP1
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcaaacctcc acgttcagct ttcgttaaat tatcaaactt acgttgagtg attaacttgg
       61 cgtttagaag ttgtctccaa tagtttttca tctttttgac tacttcttca cttggaacgt
      121 tatccgattt accacgattt ttatcagaac gcgttaagac cttattgtct attgaatcgt
      181 ctttaaggaa actttgtgga acaatggcat cgacatcata atcacttaaa cgattaatat
      241 ctaattcttg gtccacatac atgtctcttc cattttggag ataatagaga tagagctttt
      301 cattttgcaa ttgagtattt tcaacaggat gctctttaag aatctgactt cctaattctt
      361 tgataccttc ttcgattcgt ttcatacgct ctcgcgaatt tttctggccc ttttgagttg
      421 tctgattttc acgtgccatt tcaataacga tattttctgg cttatgccgc cccattactt
      481 tgaccaattc atcaacaact tttacagtct gtaaaatacc ttttttaata gcagggctac
      541 cagctaaatt tgcaatatgt tcatgtaaac tatcgccttg tccagacact tgtgcttttt
      601 gaatgtcttc tttaaatgtc aaactatcat catggatcag ctgcataaaa ttgcgattgg
      661 caaaaccatc tgatttcaaa aaatctaata ttgttttgcc agattgctta tccctaatac
      721 cattaatcaa ttttcgagac aaacgtcccc aaccagtata acggcgacgt ttaagctgtt
      781 tcatcacctt atcatcaaag aggtgagcat atgttttaag tctttcctca atcatctccc
      841 tatcttcaaa taaggtcaat gttaaaacaa tatcctctaa gatatcttca ttttcttcat
      901 tatccaaaaa atctt
//
