LOCUS       sequence-152578-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, 
            Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
  TITLE     Targeted nucleotide editing using hybrid prokaryotic and vertebrate 
            adaptive immune systems.
  JOURNAL   Science. 2016 Aug 4. pii: aaf8729.
  PUBMED    27492474
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 4. pii: aaf8729."
COMMENT     Sequence Label: pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     190..212
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     204..221
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     205..221
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     231..250
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        231..249
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     409..428
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        518..593
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
       61 cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
      121 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
      181 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
      241 cactataggg cgaattgggt accgggccaa ttcgaacgct gacgtcatca acccgctcca
      301 aggaatcgcg ggcccagtgt cactaggcgg gaacacccag cgcgcgtgcg ccctggcagg
      361 aagatggctg tgagggacag gggagtggcg ccctgcaata tttgcatgtc gctatgtgtt
      421 ctgggaaatc accataaacg tgaaatgtct ttggatttgg gaatcttata agttctgtat
      481 gaggaccaca gatccccccg agatgtcatg aaagagagtt ttagagctag aaatagcaag
      541 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt
      601 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      661 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      721 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      781 atagggactt tccatt
//
