LOCUS       sequence-152580-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, 
            Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
  TITLE     Targeted nucleotide editing using hybrid prokaryotic and vertebrate 
            adaptive immune systems.
  JOURNAL   Science. 2016 Aug 4. pii: aaf8729.
  PUBMED    27492474
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 4. pii: aaf8729."
COMMENT     Sequence Label: pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     187..209
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     201..218
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     202..218
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     228..247
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        228..246
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     406..425
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        515..590
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
       61 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
      121 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      181 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac
      241 tatagggcga attgggtacc gggccaattc gaacgctgac gtcatcaacc cgctccaagg
      301 aatcgcgggc ccagtgtcac taggcgggaa cacccagcgc gcgtgcgccc tggcaggaag
      361 atggctgtga gggacagggg agtggcgccc tgcaatattt gcatgtcgct atgtgttctg
      421 ggaaatcacc ataaacgtga aatgtctttg gatttgggaa tcttataagt tctgtatgag
      481 gaccacagat ccccccgaga tgtcatgaaa gagagtttta gagctagaaa tagcaagtta
      541 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttacg
      601 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
      661 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      721 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      781 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      841 catcaagtgt atcatatgcc
//
