LOCUS       sequence-152581-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, 
            Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
  TITLE     Targeted nucleotide editing using hybrid prokaryotic and vertebrate 
            adaptive immune systems.
  JOURNAL   Science. 2016 Aug 4. pii: aaf8729.
  PUBMED    27492474
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 4. pii: aaf8729."
COMMENT     Sequence Label: pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             94..114
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagcgaaatg gcaccgaaga agaagcgtaa agtcggaatc
      121 cacggagttc ctgcggcaat ggacaagaag tactccattg ggctcgctat cggcacaaac
      181 agcgtcggtt gggccgtcat tacggacgag tacaaggtgc cgagcaaaaa attcaaagtt
      241 ctgggcaata ccgatcgcca cagcataaag aagaacctca ttggcgccct cctgttcgac
      301 tccggggaga cggccgaagc cacgcggctc aaaagaacag cacggcgcag atatacccgc
      361 agaaagaatc ggatctgcta cctgcaggag atctttagta atgagatggc taaggtggat
      421 gactctttct tccataggct ggaggagtcc tttttggtgg aggaggataa aaagcacgag
      481 cgccacccaa tctttggcaa tatcgtggac gaggtggcgt accatgaaaa gtacccaacc
      541 atatatcatc tgaggaagaa gcttgtagac agtactgata aggctgactt gcggttgatc
      601 tatctcgcgc tggcgcatat gatcaaattt cggggacact tcctcatcga gggggacctg
      661 aacccagaca acagcgatgt cgacaaactc tttatccaac tggttcagac ttacaatcag
      721 cttttcgaag agaacccgat caacgcatcc ggagttgacg ccaaagcaat cctgagcgct
      781 aggctgtcca aatcccggcg gctcgaaaac ctcatcgcac agctccctgg
//
