LOCUS       sequence-152598-         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for C-terminal fusion with 
            TAP (no promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB428
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(82..88,89..106,107..112))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(149..273)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(299..316)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(299..315)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(308..330)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    518..542
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgacg
       61 gatcctaact caaaatccac acattatacg agccggaagc ataaagtgta aagcctgggg
      121 tgcctaatgc ggcccctcga ggggccgcca tagtgactgg atatgttgtg ttttacagta
      181 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
      241 cgtttctcgt tcagcttttt tgtacaaact tgttgataac tctagaccca agcttggcac
      301 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
      361 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
      421 cttcccaaca gttgcgcagc ctgaatggcg aatgctagag cagcttgagc ttggatcaga
      481 ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa
      541 acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt
      601 tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta
      661 ctttgatcca ac
//
