LOCUS       sequence-152602-         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for expression of gene by 
            2xCaMV35S enhancer and omega sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB402-omega
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(86..92,93..110,111..116))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(153..277)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(374..420)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(409..429)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
       61 gacggatcct aactcaaaat ccacacatta tacgagccgg aagcataaag tgtaaagcct
      121 ggggtgccta atgcggcccc tcgaggggcc gccatagtga ctggatatgt tgtgttttac
      181 agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat
      241 tttacgtttc tcgttcagct tttttgtaca aacttgttga taactctaga aattgtaaat
      301 agtaattgta atgttgtttg ttgtttgttg ttgttggtaa ttgttgtaaa aatacactgg
      361 tgatttcagc gtgtcctctc caaatgaaat gaacttcctt atatagagga agggtcttgc
      421 gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact
      481 tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt
      541 ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa
      601 tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata
      661 gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgtt
//
