LOCUS       sequence-152604-         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for C-terminal fusion with 
            GST (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB523
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(81..87,88..105,106..111))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(148..272)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(294..639)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(331..351)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgacgg
       61 atcctaactc aaaatccaca cattatacga gccggaagca taaagtgtaa agcctggggt
      121 gcctaatgcg gcccctcgag gggccgccat agtgactgga tatgttgtgt tttacagtat
      181 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac
      241 gtttctcgtt cagctttttt gtacaaactt gttgataact ctagagtccc ccgtgttctc
      301 tccaaatgaa atgaacttcc ttatatagag gaagggtctt gcgaaggata gtgggattgt
      361 gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt
      421 ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg
      481 tcggcagagg catcttcaac gatggccttt cctttatcgc aatgatggca tttgtaggag
      541 ccaccttcct tttccactat cttcacaata aagtgacaga tagctgggca atggaatccg
      601 aggaggtttc cggatattac cctttgttga aaagtctcaa ttgccctttg gtcttctgag
      661 actgtatctt tgatattttt ggagtagaca agtgtgtcgt gctccaccat g
//
