LOCUS       sequence-152615-        7509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zic2a promoter driving HA-tagged biotin ligase (BirA), a Thosea 
            asigna virus peptide (2A), and membrane-tethered mCherry protein 
            (membCherry); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7509)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 7509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-zic2a-cytoBirA-2A-mCherry_Ras
FEATURES             Location/Qualifiers
     source          1..7509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      353..941
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     842..861
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1095..1112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1229..1250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1265..1270,1271..1288,1289..1295)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1303..1319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1308..1330
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1327..1343
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1327..1343
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1362..1382
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1364..1382
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1419..1435
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1419..1435
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(1469..1480,1481..1668)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     protein_bind    1688..1712
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3265..3289)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3454..3465)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     promoter        3527..3545
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3527..3544
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      3644..3653
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3659..3685
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             3692..3718
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             3725..3751
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             3767..4729
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             3767..4729
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             4745..4798
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4805..5512
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4950..4968)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5218..5237
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(5629..5647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    5654..5788
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5678..5697)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5732..5751
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(5880..6017,6018..6029)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(6087..6104)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6087..6103)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6096..6118)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6245..6700
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6332..6351)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6542..6563
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6726..6830
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6831..6899,6900..7509,1..182)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7049..7068)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
       61 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
      121 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
      181 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
      241 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
      301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
      361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
      481 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
      541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      961 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     1021 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     1081 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     1141 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     1201 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     1261 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     1321 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     1381 gggaacaaaa gctggagctc caccgcggtg gcggccgctc tagaactagt ggatctgctg
     1441 ggcttgctga aggtaggggg tcaagaacca gaggtgtaaa gtacttgagt aattttactt
     1501 gattactgta cttaagtatt atttttgggg atttttactt tacttgagta caattaaaaa
     1561 tcaatacttt tacttttact taattacatt tttttagaaa aaaaagtact ttttactcct
     1621 tacaatttta tttacagtca aaaagtactt attttttgga gatcacttgg gcccggctcg
     1681 agatatcacc actttgtaca agaaagctgg gtcactggac ccacatattc ctcgtgttcc
     1741 agtgccagtg tacctgaaca ttaacaatac ccctgtctcg cctccccttc ttgtcttttt
     1801 cacccctttc tggtctcatt actttgggtt ttatgcccta atctacacaa tgaggacgaa
     1861 gtcatggtgg ttatcttcag cagccaatgc ctgcctacct gtaatccttc ccaaagtccc
     1921 tggctttctc acagctcaca aatcggcaca caatgctttc atgcaaggcc tctgccgtaa
     1981 tgctcaaaga cgacagcaga tttttttctt ttctcatccc ttcacatcaa atcccaccct
     2041 tcctctgaaa aacagggacg tcttgatcta agtctcagga caaggaaatg ttagaatata
     2101 aaggacacaa gagataaatg taagtttctc cagatacctc agcacctctg ttacataaac
     2161 ggaagtaaag taaaactcct gctgcactga cactgttata tatatgagca gaatgatacg
     2221 gttggttata aatgacactt aaaaaaatca atgaatttat cattaaaagt taattgagaa
     2281 agagctcaaa aaggtcaagc cagttgctcc atttgaggag ggcataaagg ggttaggttt
     2341 taacttagca tttttattga ctctgccaaa acaaaatgag tgggctctgt aggattgtgc
     2401 actctgcatt ggccattcag gccttcagag ccccattgta gtactttgaa gagaatgaaa
     2461 ggaaacccca ctggtggaaa tgcactggaa cagccccaca acaggccaca actgttccct
     2521 tatgcatgaa aggcagagac ataaaagaca agatggactt ctgaggtagg gccaaaaaca
     2581 aaggcaacaa aagtgctgat catgagggga tgacacaact cgtctcagca gaaggagcag
     2641 ggagaaaagg ggaaggggac aatggcactc ccggccaaat gacatgttga agaattgaaa
     2701 aatccacaca agaagtgtct tcttaattca ctaaacccac acgactttaa ggactgtcgg
     2761 ctggccccgg ttgcaccgag gggggtggac tgtaacagcc tttggctttt gctccccaac
     2821 tttttgctgc tgatatttgg ccgtttttgg caaacaactt ggcaaggtca gagacagact
     2881 ggggtggatg ttttagtgtg cacacaccaa acccaaccca acccaaacca aacctaatca
     2941 aagcatgcag ggttccgctc ccgcagcttc cgttttgatc tgttggctgt ctgcgtgtct
     3001 gatctgtttc tgtttgtgtc ccaaacacaa ttatgctact gcctaacctc catggaaaca
     3061 gtttgatgga caaaaacatg tttgctctca ttttaaaagg attcttgttt tgagggacac
     3121 agtaatgact gctaaatttt tgggccaaca tttcactaaa catttggtta aagcaaaaaa
     3181 gcaacttcct cttgactttc acattgctga atagaataat aataaaagca cacttacata
     3241 aaagctaccg caatgttttc cattagcctg cttttttgta caaacttgtg atatccagtg
     3301 acgtaggaag tccatccatt cacagcgctt ctataaaggc gccagctgag gcgcctacta
     3361 ctccaaccgc gactgcagcg agcaactgag aagactggat agagccggcg gttccgcgaa
     3421 cgagcagtga ccgcgctccc acccagctct gctctgcagc tcccaccagt gtctacccct
     3481 ggaccccttg ccgggctttc cccaaacttc gaggatccac cggcctattt aggtgacact
     3541 atagaagagt acaagctact tgttcttttt gcaggatccc atcgattcga attcaaggtt
     3601 aagcggccgc aagctagatt ggcattccgg tactgttggt aaagccacca tggccaccta
     3661 tgacgtccca gattacgcaa gtttgcccgg gtatgatgtt cctgattatg ctagcctccc
     3721 gggttacgat gtgcccgact atgcctcact tccaggcaga tctcctatga aggataacac
     3781 cgtgccactg aaattgattg ccctgttagc gaacggtgaa tttcactctg gcgagcagtt
     3841 gggtgaaacg ctgggaatga gccgggcggc tattaataaa cacattcaga cactgcgtga
     3901 ctggggcgtt gatgtcttta ccgttccggg taaaggatac agcctgcctg agcctatcca
     3961 gttacttaat gctaaacaga tattgggtca gctggatggc ggtagtgtag ccgtgctgcc
     4021 agtgattgac tccacgaatc agtaccttct tgatcgtatc ggagagctta aatcgggcga
     4081 tgcttgcatt gcagaatacc agcaggctgg ccgtggtcgc cggggtcgga aatggttttc
     4141 gccttttggc gcaaacttat atttgtcgat gttctggcgt ctggaacaag gcccggcggc
     4201 ggcgattggt ttaagtctgg ttatcggtat cgtgatggcg gaagtattac gcaagctggg
     4261 tgcagataaa gttcgtgtta aatggcctaa tgacctctat ctgcaggatc gcaagctggc
     4321 aggcattctg gtggagctga ctggcaaaac tggcgatgcg gcgcaaatag tcattggagc
     4381 cgggatcaac atggcaatgc gccgtgttga agagagtgtc gttaatcagg ggtggatcac
     4441 gctgcaggaa gcggggatca atctcgatcg taatacgttg gcggccatgc taatacgtga
     4501 attacgtgct gcgttggaac tcttcgaaca agaaggattg gcaccttatc tgtcgcgctg
     4561 ggaaaagctg gataatttta ttaatcgccc agtgaaactt atcattggtg ataaagaaat
     4621 atttggcatt tcacgcggaa tagacaaaca gggggcttta ttacttgagc aggatggaat
     4681 aataaaaccc tggatgggcg gtgaaatatc cctgcgtagt gcagaaaaag atatcggatc
     4741 aggagagggc aggggaagtc ttctaacatg cggggacgtg gaggaaaatc ccgggcccag
     4801 atctatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     4861 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     4921 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     4981 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     5041 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     5101 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     5161 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     5221 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     5281 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     5341 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     5401 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     5461 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agggaggagg
     5521 aagatctaag ctgaaccctc ctgatgagag tggccccggc tgcatgagct gcaagtgtgt
     5581 gctctcctga tctagaacta tagtgagtcg tattacgtat ccggactccc tatagtgagt
     5641 cgtattaacc ggtgatccag acatgataag atacattgat gagtttggac aaaccacaac
     5701 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     5761 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     5821 ggttcagggg gaggtgtggg aggttttttg atatcccggg tttaaacgca tgcagatcta
     5881 atactcaagt acaattttaa tggagtactt ttttactttt actcaagtaa gattctagcc
     5941 agatactttt acttttaatt gagtaaaatt ttccctaagt acttgtactt tcacttgagt
     6001 aaaatttttg agtacttttt acacctctgt caagaaccat atgccggtac ccaatcgccc
     6061 tataggacgt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     6121 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     6181 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     6241 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     6301 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     6361 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     6421 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     6481 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     6541 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     6601 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     6661 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     6721 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     6781 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     6841 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     6901 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     6961 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     7021 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     7081 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     7141 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     7201 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     7261 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     7321 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     7381 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     7441 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     7501 cggctggct
//
