LOCUS       sequence-152617-        7536 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zic2a promoter driving HA-tagged biotin ligase (BirA) with NLS, a 
            Thosea asigna virus peptide (2A), and membrane-tethered mCherry 
            protein (membCherry); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7536)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 7536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-zic2a-NLS-BirA-2A-mCherry_Ras
FEATURES             Location/Qualifiers
     source          1..7536
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      353..941
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     842..861
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1095..1112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1229..1250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1265..1270,1271..1288,1289..1295)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1303..1319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1308..1330
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1327..1343
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1327..1343
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1362..1382
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1364..1382
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1419..1435
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1419..1435
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(1469..1480,1481..1668)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     protein_bind    1688..1712
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3265..3289)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3454..3465)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     promoter        3527..3545
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3527..3544
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      3644..3653
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3659..3685
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             3692..3718
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             3725..3751
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             3767..3787
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3794..4756
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             3794..4756
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             4772..4825
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4832..5539
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4977..4995)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5245..5264
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(5656..5674)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    5681..5815
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5705..5724)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5759..5778
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(5907..6044,6045..6056)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(6114..6131)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6114..6130)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6123..6145)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6272..6727
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6359..6378)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6569..6590
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6753..6857
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6858..6926,6927..7536,1..182)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7076..7095)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
       61 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
      121 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
      181 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
      241 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
      301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
      361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
      481 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
      541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      961 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     1021 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     1081 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     1141 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     1201 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     1261 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     1321 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     1381 gggaacaaaa gctggagctc caccgcggtg gcggccgctc tagaactagt ggatctgctg
     1441 ggcttgctga aggtaggggg tcaagaacca gaggtgtaaa gtacttgagt aattttactt
     1501 gattactgta cttaagtatt atttttgggg atttttactt tacttgagta caattaaaaa
     1561 tcaatacttt tacttttact taattacatt tttttagaaa aaaaagtact ttttactcct
     1621 tacaatttta tttacagtca aaaagtactt attttttgga gatcacttgg gcccggctcg
     1681 agatatcacc actttgtaca agaaagctgg gtcactggac ccacatattc ctcgtgttcc
     1741 agtgccagtg tacctgaaca ttaacaatac ccctgtctcg cctccccttc ttgtcttttt
     1801 cacccctttc tggtctcatt actttgggtt ttatgcccta atctacacaa tgaggacgaa
     1861 gtcatggtgg ttatcttcag cagccaatgc ctgcctacct gtaatccttc ccaaagtccc
     1921 tggctttctc acagctcaca aatcggcaca caatgctttc atgcaaggcc tctgccgtaa
     1981 tgctcaaaga cgacagcaga tttttttctt ttctcatccc ttcacatcaa atcccaccct
     2041 tcctctgaaa aacagggacg tcttgatcta agtctcagga caaggaaatg ttagaatata
     2101 aaggacacaa gagataaatg taagtttctc cagatacctc agcacctctg ttacataaac
     2161 ggaagtaaag taaaactcct gctgcactga cactgttata tatatgagca gaatgatacg
     2221 gttggttata aatgacactt aaaaaaatca atgaatttat cattaaaagt taattgagaa
     2281 agagctcaaa aaggtcaagc cagttgctcc atttgaggag ggcataaagg ggttaggttt
     2341 taacttagca tttttattga ctctgccaaa acaaaatgag tgggctctgt aggattgtgc
     2401 actctgcatt ggccattcag gccttcagag ccccattgta gtactttgaa gagaatgaaa
     2461 ggaaacccca ctggtggaaa tgcactggaa cagccccaca acaggccaca actgttccct
     2521 tatgcatgaa aggcagagac ataaaagaca agatggactt ctgaggtagg gccaaaaaca
     2581 aaggcaacaa aagtgctgat catgagggga tgacacaact cgtctcagca gaaggagcag
     2641 ggagaaaagg ggaaggggac aatggcactc ccggccaaat gacatgttga agaattgaaa
     2701 aatccacaca agaagtgtct tcttaattca ctaaacccac acgactttaa ggactgtcgg
     2761 ctggccccgg ttgcaccgag gggggtggac tgtaacagcc tttggctttt gctccccaac
     2821 tttttgctgc tgatatttgg ccgtttttgg caaacaactt ggcaaggtca gagacagact
     2881 ggggtggatg ttttagtgtg cacacaccaa acccaaccca acccaaacca aacctaatca
     2941 aagcatgcag ggttccgctc ccgcagcttc cgttttgatc tgttggctgt ctgcgtgtct
     3001 gatctgtttc tgtttgtgtc ccaaacacaa ttatgctact gcctaacctc catggaaaca
     3061 gtttgatgga caaaaacatg tttgctctca ttttaaaagg attcttgttt tgagggacac
     3121 agtaatgact gctaaatttt tgggccaaca tttcactaaa catttggtta aagcaaaaaa
     3181 gcaacttcct cttgactttc acattgctga atagaataat aataaaagca cacttacata
     3241 aaagctaccg caatgttttc cattagcctg cttttttgta caaacttgtg atatccagtg
     3301 acgtaggaag tccatccatt cacagcgctt ctataaaggc gccagctgag gcgcctacta
     3361 ctccaaccgc gactgcagcg agcaactgag aagactggat agagccggcg gttccgcgaa
     3421 cgagcagtga ccgcgctccc acccagctct gctctgcagc tcccaccagt gtctacccct
     3481 ggaccccttg ccgggctttc cccaaacttc gaggatccac cggcctattt aggtgacact
     3541 atagaagagt acaagctact tgttcttttt gcaggatccc atcgattcga attcaaggtt
     3601 aagcggccgc aagctagatt ggcattccgg tactgttggt aaagccacca tggccaccta
     3661 tgacgtccca gattacgcaa gtttgcccgg gtatgatgtt cctgattatg ctagcctccc
     3721 gggttacgat gtgcccgact atgcctcact tccaggcaga tctcctccaa aaaagaagag
     3781 aaaggtacga tctatgaagg ataacaccgt gccactgaaa ttgattgccc tgttagcgaa
     3841 cggtgaattt cactctggcg agcagttggg tgaaacgctg ggaatgagcc gggcggctat
     3901 taataaacac attcagacac tgcgtgactg gggcgttgat gtctttaccg ttccgggtaa
     3961 aggatacagc ctgcctgagc ctatccagtt acttaatgct aaacagatat tgggtcagct
     4021 ggatggcggt agtgtagccg tgctgccagt gattgactcc acgaatcagt accttcttga
     4081 tcgtatcgga gagcttaaat cgggcgatgc ttgcattgca gaataccagc aggctggccg
     4141 tggtcgccgg ggtcggaaat ggttttcgcc ttttggcgca aacttatatt tgtcgatgtt
     4201 ctggcgtctg gaacaaggcc cggcggcggc gattggttta agtctggtta tcggtatcgt
     4261 gatggcggaa gtattacgca agctgggtgc agataaagtt cgtgttaaat ggcctaatga
     4321 cctctatctg caggatcgca agctggcagg cattctggtg gagctgactg gcaaaactgg
     4381 cgatgcggcg caaatagtca ttggagccgg gatcaacatg gcaatgcgcc gtgttgaaga
     4441 gagtgtcgtt aatcaggggt ggatcacgct gcaggaagcg gggatcaatc tcgatcgtaa
     4501 tacgttggcg gccatgctaa tacgtgaatt acgtgctgcg ttggaactct tcgaacaaga
     4561 aggattggca ccttatctgt cgcgctggga aaagctggat aattttatta atcgcccagt
     4621 gaaacttatc attggtgata aagaaatatt tggcatttca cgcggaatag acaaacaggg
     4681 ggctttatta cttgagcagg atggaataat aaaaccctgg atgggcggtg aaatatccct
     4741 gcgtagtgca gaaaaagata tcggatcagg agagggcagg ggaagtcttc taacatgcgg
     4801 ggacgtggag gaaaatcccg ggcccagatc tatggtgagc aagggcgagg aggataacat
     4861 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     4921 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     4981 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     5041 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     5101 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     5161 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     5221 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     5281 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     5341 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     5401 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     5461 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     5521 catggacgag ctgtacaagg gaggaggaag atctaagctg aaccctcctg atgagagtgg
     5581 ccccggctgc atgagctgca agtgtgtgct ctcctgatct agaactatag tgagtcgtat
     5641 tacgtatccg gactccctat agtgagtcgt attaaccggt gatccagaca tgataagata
     5701 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     5761 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     5821 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttgata
     5881 tcccgggttt aaacgcatgc agatctaata ctcaagtaca attttaatgg agtacttttt
     5941 tacttttact caagtaagat tctagccaga tacttttact tttaattgag taaaattttc
     6001 cctaagtact tgtactttca cttgagtaaa atttttgagt actttttaca cctctgtcaa
     6061 gaaccatatg ccggtaccca atcgccctat aggacgtcgt attacgcgcg ctcactggcc
     6121 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     6181 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     6241 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     6301 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     6361 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6421 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6481 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6541 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6601 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     6661 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     6721 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     6781 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     6841 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     6901 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     6961 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     7021 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7081 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     7141 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     7201 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     7261 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     7321 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     7381 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7441 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     7501 caggaccact tctgcgctcg gcccttccgg ctggct
//
