LOCUS       sequence-152619-        8365 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a 
            Thosea asigna virus peptide (2A), and membrane-tethered mCherry 
            protein (membCherry); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 8365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMTB2-cytoBirA-2A-mCherry_Ras
FEATURES             Location/Qualifiers
     source          1..8365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      353..941
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     842..861
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1095..1112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1229..1250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1265..1270,1271..1288,1289..1295)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1303..1319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1308..1330
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1327..1343
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1327..1343
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1362..1382
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1364..1382
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1419..1435
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1419..1435
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(1469..1480,1481..1668)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     promoter        4383..4401
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4383..4400
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      4500..4509
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4515..4541
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             4548..4574
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             4581..4607
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             4623..5585
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             4623..5585
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             5601..5654
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5661..6368
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5806..5824)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6074..6093
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(6485..6503)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    6510..6644
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6534..6553)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6588..6607
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(6736..6873,6874..6885)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(6943..6960)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6943..6959)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6952..6974)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7101..7556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7188..7207)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7398..7419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7582..7686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7687..7755,7756..8365,1..182)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7905..7924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
       61 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
      121 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
      181 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
      241 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
      301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
      361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
      481 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
      541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      961 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     1021 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     1081 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     1141 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     1201 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     1261 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     1321 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     1381 gggaacaaaa gctggagctc caccgcggtg gcggccgctc tagaactagt ggatctgctg
     1441 ggcttgctga aggtaggggg tcaagaacca gaggtgtaaa gtacttgagt aattttactt
     1501 gattactgta cttaagtatt atttttgggg atttttactt tacttgagta caattaaaaa
     1561 tcaatacttt tacttttact taattacatt tttttagaaa aaaaagtact ttttactcct
     1621 tacaatttta tttacagtca aaaagtactt attttttgga gatcacttgg gcccggctcg
     1681 agggcctagt cattacctat actcaacgtt tactctgaga aacttccata ttttagaggt
     1741 aaaatgctca gaattgtaaa aagtcttgtc aaaatacttg gaatttgcca aaaacaaaat
     1801 aaaagtcaca tcacctcatt gtccttggta tcagcaagca cttttaacac aatcagctgt
     1861 ttttgtttat aactcccact gaaggccatg tgcattactc ccaagttaca cagtaaaaag
     1921 tcacatgtag tttatcatga tcactgctgc tcaagcacag ctttatctgt tattcagtgt
     1981 ggcaatatct gaaatgtgtg caaaatatta tttattgtag ctgatcacca acactttcag
     2041 tgtttttttt ttctactttt aatgagaatt atgttcttag gatttatcct tataaacctc
     2101 tttaaaaaga aatgttatgt ctgcatagac acgacatgaa attatgaaca gaaaatttaa
     2161 ctatttaaga tcaagacttg acatataaca tttctaatga acattaaacc tgaggtaggc
     2221 ccacttgcaa aaatgtaact gctatcactc actttataca tcattgtgta acttgtacac
     2281 acaaaaatag tgaagcaatg tggcatggaa tgcaggtccc attcattcaa agttattaaa
     2341 gcactttctt aaagtgcatt actactgagc taagaccagc tagttgctca tacaacatac
     2401 tacaagtagt gtcatcctaa tttggataaa aaaaatgtcc attttataca tggaaaagta
     2461 ctcattagat cccccattgt ttgtattacg gtatttcgtg aacacaagag gtaaatgacc
     2521 tacagagctg ctgttgtgtt agattgaaaa cacaacacag gatcatggag gcatccatca
     2581 ttatgacgca ttctgccact tgtaactctg cacagacttt ttgaaaagtt taattgagtc
     2641 ttggcgttgt cgcagaaaaa tacgaaaaat tctgtgttaa aatttgcaaa taaaaattcc
     2701 agttttatag gaaatcgcca tggccttgtt ctttaaccag ttaagcttcc ccttctttca
     2761 ctctcaagtt gcaagaagca agtgtagcaa tgtgcacgcg acagccgggt gtgtgacgct
     2821 ggaccaatca gagcacagag ctccgaaagt ttacctttta tggctagagc cgggtatgtg
     2881 ccgtcatata aaagagcgcg cccagctttt cagcctcact ttgagctcct ccacacgcag
     2941 ctagtgcgga atatcatctg cttgtaaccc attctcttaa gtcgacaacc ccccaaaccc
     3001 aaggtgagtt ggttttacag tttttaagcg tttaattata gtttctatct attaatgtta
     3061 agtaaaatgt gtaaatggat aaatatgatt tgatgaagtc tttagacagt tcataacggt
     3121 ctctttgtag ttatctacta ggtttgattt ataagtggtg gttcttttga taatctatgt
     3181 tacatttaat aaatattggc tgtatcttaa cttttttgta gcgttagtgg atgtggcagg
     3241 tgagaatgtc ggtaatttaa cgtgaccagt cgtaggcacg acattgaaca ttgaataggc
     3301 cggtgtgaaa tgggtgttaa gtcttaatat aaagttgtgt tctggctaat ctctctttaa
     3361 cagccataaa tgtgattttt aactacagtt ttgagggtgc tggtgagcat gttgcacact
     3421 tgatggatag ccggcatggg aagttctttg tgcaggcagt gctgcagcag ggtgtgacct
     3481 actttagcta gccggctaac cagctctcat ctgatgtaac gtaaacccca tcaatctaac
     3541 atcggctcat tcgtgcttta taagatcgaa gatcttgtta ttagtaacgg gtattggcat
     3601 ttagttttaa cttggttaat tttctttttg ttatgtgttc tatttaaatg tagcctgcag
     3661 ccccctctgt ctttaatgaa gctgaagatt aacgttaacg ttagtgaagt gttgtatccg
     3721 ctatgaaaga tttgaatttt agaggtgtgg tccactttaa taacggctgc ggagttctct
     3781 ggctgcactt cagccgctgt gactgtcgaa gcagagccca tcagtttttc aggatctcgg
     3841 tgatttttga ggctagacta tgaactgaac cgactgagag tgaccgtcca gaaatgcttt
     3901 agtgtgaagt ctccgctcac gaggagtccg tggactgact gcagatctgt gacgcagtaa
     3961 cacccggcgg cagacacccg ttgaaatgcg gttgtgtaat tgatcgcagg cgagggtgga
     4021 agaggatgtg aaacttcatt tgtgtaaaat ttagggagtg gtcctggtgt gatgaatgtc
     4081 gaaatctgtt cctttttact gagccctacg actctggctg agtgccacac cgccggcagc
     4141 cgcaaagcgg ctcaaaccat tgccttttat ggtaataatg agagaatgca gagggacttc
     4201 ctttgtctgg catatctgag gcgcgcgctg tcactagcgc ccaccagcgg tcagactgta
     4261 gaatgcagag caaaacagga agttgactcc agatggtcac atgcttgctg aaacgcctct
     4321 ccctggcagc agtgcaattc taacagtttc cctttctttt acagttcagc catcctaggc
     4381 ctatttaggt gacactatag aagagtacaa gctacttgtt ctttttgcag gatcccatcg
     4441 attcgaattc aaggttaagc ggccgcaagc tagattggca ttccggtact gttggtaaag
     4501 ccaccatggc cacctatgac gtcccagatt acgcaagttt gcccgggtat gatgttcctg
     4561 attatgctag cctcccgggt tacgatgtgc ccgactatgc ctcacttcca ggcagatctc
     4621 ctatgaagga taacaccgtg ccactgaaat tgattgccct gttagcgaac ggtgaatttc
     4681 actctggcga gcagttgggt gaaacgctgg gaatgagccg ggcggctatt aataaacaca
     4741 ttcagacact gcgtgactgg ggcgttgatg tctttaccgt tccgggtaaa ggatacagcc
     4801 tgcctgagcc tatccagtta cttaatgcta aacagatatt gggtcagctg gatggcggta
     4861 gtgtagccgt gctgccagtg attgactcca cgaatcagta ccttcttgat cgtatcggag
     4921 agcttaaatc gggcgatgct tgcattgcag aataccagca ggctggccgt ggtcgccggg
     4981 gtcggaaatg gttttcgcct tttggcgcaa acttatattt gtcgatgttc tggcgtctgg
     5041 aacaaggccc ggcggcggcg attggtttaa gtctggttat cggtatcgtg atggcggaag
     5101 tattacgcaa gctgggtgca gataaagttc gtgttaaatg gcctaatgac ctctatctgc
     5161 aggatcgcaa gctggcaggc attctggtgg agctgactgg caaaactggc gatgcggcgc
     5221 aaatagtcat tggagccggg atcaacatgg caatgcgccg tgttgaagag agtgtcgtta
     5281 atcaggggtg gatcacgctg caggaagcgg ggatcaatct cgatcgtaat acgttggcgg
     5341 ccatgctaat acgtgaatta cgtgctgcgt tggaactctt cgaacaagaa ggattggcac
     5401 cttatctgtc gcgctgggaa aagctggata attttattaa tcgcccagtg aaacttatca
     5461 ttggtgataa agaaatattt ggcatttcac gcggaataga caaacagggg gctttattac
     5521 ttgagcagga tggaataata aaaccctgga tgggcggtga aatatccctg cgtagtgcag
     5581 aaaaagatat cggatcagga gagggcaggg gaagtcttct aacatgcggg gacgtggagg
     5641 aaaatcccgg gcccagatct atggtgagca agggcgagga ggataacatg gccatcatca
     5701 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga
     5761 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     5821 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct
     5881 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg
     5941 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
     6001 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact
     6061 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc
     6121 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg
     6181 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
     6241 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca
     6301 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
     6361 tgtacaaggg aggaggaaga tctaagctga accctcctga tgagagtggc cccggctgca
     6421 tgagctgcaa gtgtgtgctc tcctgatcta gaactatagt gagtcgtatt acgtatccgg
     6481 actccctata gtgagtcgta ttaaccggtg atccagacat gataagatac attgatgagt
     6541 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     6601 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     6661 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttgatat cccgggttta
     6721 aacgcatgca gatctaatac tcaagtacaa ttttaatgga gtactttttt acttttactc
     6781 aagtaagatt ctagccagat acttttactt ttaattgagt aaaattttcc ctaagtactt
     6841 gtactttcac ttgagtaaaa tttttgagta ctttttacac ctctgtcaag aaccatatgc
     6901 cggtacccaa tcgccctata ggacgtcgta ttacgcgcgc tcactggccg tcgttttaca
     6961 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     7021 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     7081 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     7141 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     7201 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     7261 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     7321 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     7381 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     7441 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     7501 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     7561 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     7621 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     7681 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     7741 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     7801 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     7861 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     7921 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     7981 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     8041 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     8101 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     8161 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     8221 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     8281 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     8341 ctgcgctcgg cccttccggc tggct
//
