LOCUS       sequence-152621-        8392 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Beta-actin2 promoter driving HA-tagged biotin ligase (BirA) with 
            NLS, a Thosea asigna virus peptide (2A), and membrane-tethered 
            mCherry protein (membCherry); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8392)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 8392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMTB2-NLS-BirA-2A-mCherry_Ras
FEATURES             Location/Qualifiers
     source          1..8392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      353..941
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     842..861
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1095..1112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1229..1250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1265..1270,1271..1288,1289..1295)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1303..1319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1308..1330
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1327..1343
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1327..1343
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1362..1382
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1364..1382
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1419..1435
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1419..1435
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(1469..1480,1481..1668)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     promoter        4383..4401
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4383..4400
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      4500..4509
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4515..4541
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             4548..4574
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             4581..4607
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             4623..4643
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4650..5612
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             4650..5612
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             5628..5681
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5688..6395
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5833..5851)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6101..6120
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(6512..6530)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    6537..6671
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6561..6580)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6615..6634
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(6763..6900,6901..6912)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(6970..6987)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6970..6986)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6979..7001)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7128..7583
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7215..7234)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7425..7446
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7609..7713
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7714..7782,7783..8392,1..182)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7932..7951)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
       61 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
      121 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
      181 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
      241 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
      301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
      361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
      481 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
      541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      961 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     1021 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     1081 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     1141 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     1201 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     1261 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     1321 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     1381 gggaacaaaa gctggagctc caccgcggtg gcggccgctc tagaactagt ggatctgctg
     1441 ggcttgctga aggtaggggg tcaagaacca gaggtgtaaa gtacttgagt aattttactt
     1501 gattactgta cttaagtatt atttttgggg atttttactt tacttgagta caattaaaaa
     1561 tcaatacttt tacttttact taattacatt tttttagaaa aaaaagtact ttttactcct
     1621 tacaatttta tttacagtca aaaagtactt attttttgga gatcacttgg gcccggctcg
     1681 agggcctagt cattacctat actcaacgtt tactctgaga aacttccata ttttagaggt
     1741 aaaatgctca gaattgtaaa aagtcttgtc aaaatacttg gaatttgcca aaaacaaaat
     1801 aaaagtcaca tcacctcatt gtccttggta tcagcaagca cttttaacac aatcagctgt
     1861 ttttgtttat aactcccact gaaggccatg tgcattactc ccaagttaca cagtaaaaag
     1921 tcacatgtag tttatcatga tcactgctgc tcaagcacag ctttatctgt tattcagtgt
     1981 ggcaatatct gaaatgtgtg caaaatatta tttattgtag ctgatcacca acactttcag
     2041 tgtttttttt ttctactttt aatgagaatt atgttcttag gatttatcct tataaacctc
     2101 tttaaaaaga aatgttatgt ctgcatagac acgacatgaa attatgaaca gaaaatttaa
     2161 ctatttaaga tcaagacttg acatataaca tttctaatga acattaaacc tgaggtaggc
     2221 ccacttgcaa aaatgtaact gctatcactc actttataca tcattgtgta acttgtacac
     2281 acaaaaatag tgaagcaatg tggcatggaa tgcaggtccc attcattcaa agttattaaa
     2341 gcactttctt aaagtgcatt actactgagc taagaccagc tagttgctca tacaacatac
     2401 tacaagtagt gtcatcctaa tttggataaa aaaaatgtcc attttataca tggaaaagta
     2461 ctcattagat cccccattgt ttgtattacg gtatttcgtg aacacaagag gtaaatgacc
     2521 tacagagctg ctgttgtgtt agattgaaaa cacaacacag gatcatggag gcatccatca
     2581 ttatgacgca ttctgccact tgtaactctg cacagacttt ttgaaaagtt taattgagtc
     2641 ttggcgttgt cgcagaaaaa tacgaaaaat tctgtgttaa aatttgcaaa taaaaattcc
     2701 agttttatag gaaatcgcca tggccttgtt ctttaaccag ttaagcttcc ccttctttca
     2761 ctctcaagtt gcaagaagca agtgtagcaa tgtgcacgcg acagccgggt gtgtgacgct
     2821 ggaccaatca gagcacagag ctccgaaagt ttacctttta tggctagagc cgggtatgtg
     2881 ccgtcatata aaagagcgcg cccagctttt cagcctcact ttgagctcct ccacacgcag
     2941 ctagtgcgga atatcatctg cttgtaaccc attctcttaa gtcgacaacc ccccaaaccc
     3001 aaggtgagtt ggttttacag tttttaagcg tttaattata gtttctatct attaatgtta
     3061 agtaaaatgt gtaaatggat aaatatgatt tgatgaagtc tttagacagt tcataacggt
     3121 ctctttgtag ttatctacta ggtttgattt ataagtggtg gttcttttga taatctatgt
     3181 tacatttaat aaatattggc tgtatcttaa cttttttgta gcgttagtgg atgtggcagg
     3241 tgagaatgtc ggtaatttaa cgtgaccagt cgtaggcacg acattgaaca ttgaataggc
     3301 cggtgtgaaa tgggtgttaa gtcttaatat aaagttgtgt tctggctaat ctctctttaa
     3361 cagccataaa tgtgattttt aactacagtt ttgagggtgc tggtgagcat gttgcacact
     3421 tgatggatag ccggcatggg aagttctttg tgcaggcagt gctgcagcag ggtgtgacct
     3481 actttagcta gccggctaac cagctctcat ctgatgtaac gtaaacccca tcaatctaac
     3541 atcggctcat tcgtgcttta taagatcgaa gatcttgtta ttagtaacgg gtattggcat
     3601 ttagttttaa cttggttaat tttctttttg ttatgtgttc tatttaaatg tagcctgcag
     3661 ccccctctgt ctttaatgaa gctgaagatt aacgttaacg ttagtgaagt gttgtatccg
     3721 ctatgaaaga tttgaatttt agaggtgtgg tccactttaa taacggctgc ggagttctct
     3781 ggctgcactt cagccgctgt gactgtcgaa gcagagccca tcagtttttc aggatctcgg
     3841 tgatttttga ggctagacta tgaactgaac cgactgagag tgaccgtcca gaaatgcttt
     3901 agtgtgaagt ctccgctcac gaggagtccg tggactgact gcagatctgt gacgcagtaa
     3961 cacccggcgg cagacacccg ttgaaatgcg gttgtgtaat tgatcgcagg cgagggtgga
     4021 agaggatgtg aaacttcatt tgtgtaaaat ttagggagtg gtcctggtgt gatgaatgtc
     4081 gaaatctgtt cctttttact gagccctacg actctggctg agtgccacac cgccggcagc
     4141 cgcaaagcgg ctcaaaccat tgccttttat ggtaataatg agagaatgca gagggacttc
     4201 ctttgtctgg catatctgag gcgcgcgctg tcactagcgc ccaccagcgg tcagactgta
     4261 gaatgcagag caaaacagga agttgactcc agatggtcac atgcttgctg aaacgcctct
     4321 ccctggcagc agtgcaattc taacagtttc cctttctttt acagttcagc catcctaggc
     4381 ctatttaggt gacactatag aagagtacaa gctacttgtt ctttttgcag gatcccatcg
     4441 attcgaattc aaggttaagc ggccgcaagc tagattggca ttccggtact gttggtaaag
     4501 ccaccatggc cacctatgac gtcccagatt acgcaagttt gcccgggtat gatgttcctg
     4561 attatgctag cctcccgggt tacgatgtgc ccgactatgc ctcacttcca ggcagatctc
     4621 ctccaaaaaa gaagagaaag gtacgatcta tgaaggataa caccgtgcca ctgaaattga
     4681 ttgccctgtt agcgaacggt gaatttcact ctggcgagca gttgggtgaa acgctgggaa
     4741 tgagccgggc ggctattaat aaacacattc agacactgcg tgactggggc gttgatgtct
     4801 ttaccgttcc gggtaaagga tacagcctgc ctgagcctat ccagttactt aatgctaaac
     4861 agatattggg tcagctggat ggcggtagtg tagccgtgct gccagtgatt gactccacga
     4921 atcagtacct tcttgatcgt atcggagagc ttaaatcggg cgatgcttgc attgcagaat
     4981 accagcaggc tggccgtggt cgccggggtc ggaaatggtt ttcgcctttt ggcgcaaact
     5041 tatatttgtc gatgttctgg cgtctggaac aaggcccggc ggcggcgatt ggtttaagtc
     5101 tggttatcgg tatcgtgatg gcggaagtat tacgcaagct gggtgcagat aaagttcgtg
     5161 ttaaatggcc taatgacctc tatctgcagg atcgcaagct ggcaggcatt ctggtggagc
     5221 tgactggcaa aactggcgat gcggcgcaaa tagtcattgg agccgggatc aacatggcaa
     5281 tgcgccgtgt tgaagagagt gtcgttaatc aggggtggat cacgctgcag gaagcgggga
     5341 tcaatctcga tcgtaatacg ttggcggcca tgctaatacg tgaattacgt gctgcgttgg
     5401 aactcttcga acaagaagga ttggcacctt atctgtcgcg ctgggaaaag ctggataatt
     5461 ttattaatcg cccagtgaaa cttatcattg gtgataaaga aatatttggc atttcacgcg
     5521 gaatagacaa acagggggct ttattacttg agcaggatgg aataataaaa ccctggatgg
     5581 gcggtgaaat atccctgcgt agtgcagaaa aagatatcgg atcaggagag ggcaggggaa
     5641 gtcttctaac atgcggggac gtggaggaaa atcccgggcc cagatctatg gtgagcaagg
     5701 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     5761 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     5821 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     5881 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     5941 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     6001 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     6061 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     6121 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     6181 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     6241 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     6301 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     6361 gccactccac cggcggcatg gacgagctgt acaagggagg aggaagatct aagctgaacc
     6421 ctcctgatga gagtggcccc ggctgcatga gctgcaagtg tgtgctctcc tgatctagaa
     6481 ctatagtgag tcgtattacg tatccggact ccctatagtg agtcgtatta accggtgatc
     6541 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     6601 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     6661 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     6721 gggaggtttt ttgatatccc gggtttaaac gcatgcagat ctaatactca agtacaattt
     6781 taatggagta cttttttact tttactcaag taagattcta gccagatact tttactttta
     6841 attgagtaaa attttcccta agtacttgta ctttcacttg agtaaaattt ttgagtactt
     6901 tttacacctc tgtcaagaac catatgccgg tacccaatcg ccctatagga cgtcgtatta
     6961 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     7021 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     7081 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     7141 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     7201 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     7261 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     7321 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     7381 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     7441 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     7501 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     7561 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     7621 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     7681 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     7741 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     7801 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     7861 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7921 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     7981 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     8041 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     8101 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     8161 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     8221 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     8281 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     8341 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ct
//
