LOCUS       sequence-152624-         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for C-terminal fusion with 
            10xMyc (no promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB519
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(81..87,88..105,106..111))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(136..260)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(286..303)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(286..302)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(295..317)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    505..529
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgacgg
       61 atcctaactc aaaatccaca cattatacga gccggaagca taaagtgtaa agcctggggt
      121 gcctaatgcg gccgccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
      181 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
      241 gcttttttgt acaaacttgt tgataactct agacccaagc ttggcactgg ccgtcgtttt
      301 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
      361 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
      421 gcgcagcctg aatggcgaat gctagagcag cttgagcttg gatcagattg tcgtttcccg
      481 ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa
      541 gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc
      601 atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc
      661 cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga
      721 catgtcgcac aagtcctaag ttacgcgaca ggc
//
