LOCUS       sequence-152626-       12124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for C-terminal fusion with 
            3xHA (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12124)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 12124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB514
FEATURES             Location/Qualifiers
     source          1..12124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1179..1303
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1338..1343,1344..1361,1362..1368)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1422..2081
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1488..1507)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2423..2728
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2607..2626
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2769..2893)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2922..3011
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      3045..3297
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3331..3347)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3331..3347)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3344..3366)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3355..3371
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3379..3385,3386..3403,3404..3409))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3424..3445
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3481..3733)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3884..4909)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(4961..5140)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5646..5670
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6191..6982
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7228..7816
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7717..7736
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7970..7987
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8002..8142
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8068..8087)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8228..8250
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8486..8680
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8746..9819)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10248..10877)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc tcgagggccg
     1321 cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga
     1381 gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
     1441 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
     1501 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
     1561 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
     1621 tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata
     1681 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
     1741 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
     1801 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
     1861 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
     1921 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
     1981 tggcgattca ggttcatcat gccgtctgtg atggcttcca tgtcggcaga atgcttaatg
     2041 aattacaaca gtactgcgat gagtggcagg gcggggcgta aagatctgga tccggcttac
     2101 taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata
     2161 ctgatatgta tacccgaagt atgtcaaaaa gaggtgtgct atgaagcagc gtattacagt
     2221 gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt
     2281 ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg
     2341 gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct
     2401 gacgagaaca gggactggtg aaatgcagtt taaggtttac acctataaaa gagagagccg
     2461 ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt
     2521 gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt
     2581 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
     2641 ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
     2701 cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc
     2761 aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat
     2821 gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct
     2881 tgtacaaagt ggttgataac agcgggttaa ttaacatctt ttacccatac gatgttcctg
     2941 actatgcggg ctatccctat gacgtcccgg actatgcagg atcctatcca tatgacgttc
     3001 cagattacgc tgctcagtgc agcgcttaga gctcgaattt ccccgatcgt tcaaacattt
     3061 ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat
     3121 ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga
     3181 gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa
     3241 tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg
     3301 aattggttcc ggaaccaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     3361 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     3421 ctaatgagtg agctaactca cattaggctg aattaggcgc gcctatttct attaattccc
     3481 gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt
     3541 tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca
     3601 taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt
     3661 atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa
     3721 tgtttgaacg atcggggaaa ttcgggggat cgatcccgcg aactgtggac gagaactgtg
     3781 ccaccaagcg taaggccgtt ctctcgcatt tgccttgcta ggctcgcgcg agttgctggc
     3841 tgaggcgttc tcgaaatcag ctcttgttcg gtcggcatct actctattcc tttgccctcg
     3901 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc
     3961 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga
     4021 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc
     4081 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg
     4141 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg
     4201 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc
     4261 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg
     4321 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag
     4381 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg
     4441 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc
     4501 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct
     4561 ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac
     4621 cctgtgcacg gcgggagatg caataggtca ggctctcgct gaatgcccca atgtcaagca
     4681 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga
     4741 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga
     4801 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt
     4861 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tcttattgcc
     4921 ccccgggatc agatctggat tgagagtgaa tatgagactc taattggata ccgaggggaa
     4981 tttatggaac gtcagtggag catttttgac aagaaatatt tgctagctga tagtgacctt
     5041 aggcgacttt tgaacgcgca ataatggttt ctgacgtatg tgcttagctc attaaactcc
     5101 agaaacccgc ggctgagtgg ctccttcaac gttgcggttc tgtcagttcc aaacgtaaaa
     5161 cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact cccttaattc tccgctcatg
     5221 atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc
     5281 agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctgtcc
     5341 ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc tgctttctct
     5401 ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc
     5461 accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg
     5521 agtgcttgcg gcagcgtgaa gctgatccgt cgagattaat agaaataaat tcagcctaat
     5581 tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc
     5641 aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag
     5701 ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg
     5761 agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca
     5821 actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac
     5881 gatgacagag cgttgctgcc tgtgatcaat tcgggcacga acccagtgga cataagcctg
     5941 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
     6001 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     6061 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     6121 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     6181 gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     6241 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     6301 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
     6361 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     6421 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     6481 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     6541 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     6601 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     6661 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     6721 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     6781 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     6841 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     6901 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
     6961 caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga cgccgcttcg
     7021 cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta
     7081 tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg
     7141 agatatatca tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7201 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7261 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7321 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     7381 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7441 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7501 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7561 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7621 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7681 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7741 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7801 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7861 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7921 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     7981 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     8041 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     8101 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     8161 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     8221 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     8281 cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc
     8341 tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg cgataggccg
     8401 acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc
     8461 ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat
     8521 aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta
     8581 catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc
     8641 aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagac cttttcgacc
     8701 tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg aaccggcgga
     8761 tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag cctgccccgc
     8821 ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc gcacggtgaa
     8881 acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct tgaacaacca
     8941 tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc
     9001 gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct tgccttctgt
     9061 gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc gcccggtttc
     9121 gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg
     9181 gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta accgaatgca
     9241 ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga acttgagtac
     9301 gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt cccggtatcg
     9361 gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat cccacacact
     9421 ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct cgtagcggat
     9481 cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt ccatgatgct
     9541 gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg gttgctcgtc
     9601 gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc gggattcttt
     9661 gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg
     9721 ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac ccagcgccgc
     9781 gccgatttgt accgggccgg atggtttgcg accgctcacg ccgattcctc gggcttgggg
     9841 gttccagtgc cattgcaggg ccggcagaca acccagccgc ttacgcctgg ccaaccgccc
     9901 gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg
     9961 ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg
    10021 tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta
    10081 catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg
    10141 cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc
    10201 acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt
    10261 gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat
    10321 tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg
    10381 gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc
    10441 gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca
    10501 atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc
    10561 tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc
    10621 tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca
    10681 atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa
    10741 tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc
    10801 agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt
    10861 acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat
    10921 acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc
    10981 ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca
    11041 aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt
    11101 acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc
    11161 cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg ccgccagggc
    11221 gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt
    11281 cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc
    11341 agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg
    11401 tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg cggcctcgcg
    11461 cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc cggcgaacac
    11521 ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg
    11581 caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg
    11641 ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat
    11701 cctggccagc tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa acagcttggt
    11761 gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt cggtgctgac
    11821 gcgggcatag cccagcaggc cagcggcggc gctcttgttc atggcgtaat gtctccggtt
    11881 ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa
    11941 gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga
    12001 cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt ggatcaaagt
    12061 actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa
    12121 acct
//
