LOCUS       sequence-152627-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for C-terminal fusion with 
            3xHA (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB514
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(81..87,88..105,106..111))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(146..270)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(292..637)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(329..349)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgacgg
       61 atcctaactc aaaatccaca cattatacga gccggaagca taaagtgtaa agcctggggt
      121 gcctaatgcg gccctcgagg gccgccatag tgactggata tgttgtgttt tacagtatta
      181 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt
      241 ttctcgttca gcttttttgt acaaacttgt tgataactct agagtccccc gtgttctctc
      301 caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt gggattgtgc
      361 gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg
      421 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
      481 ggcagaggca tcttcaacga tggcctttcc tttatcgcaa tgatggcatt tgtaggagcc
      541 accttccttt tccactatct tcacaataaa gtgacagata gctgggcaat ggaatccgag
      601 gaggtttccg gatattaccc tttgttgaaa agtctcaatt gccctttggt cttctgagac
      661 tgtatctttg atatttttgg agtagacaag tgtgtcgtgc tccaccatgt tgacgaagat
      721 tttcttcttg tcattgagtc gtaagagact ctgtatgaac tgttcgccag tctttacggc
      781 gagttctgtt aggtcctcta tttgaatctt tgactccatg gcctttgatt cagtgggaac
      841 tacct
//
