LOCUS       sequence-152631-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for C-terminal fusion with 
            eYFP (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB541
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(110..234)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(245..247,248..250,251..763)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(290..311)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(551..570)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
       61 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
      121 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
      181 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttgataa
      241 cagcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      301 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      361 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      421 caccctcgtg accaccttcg gctacggcct gcagtgcttc gcccgctacc ccgaccacat
      481 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      541 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
      601 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
      661 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
      721 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
      781 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
      841 ccactacctg agctaccagt ccgc
//
