LOCUS       sequence-152635-         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for C-terminal fusion with 
            T7 (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB526
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(86..92,93..110,111..116))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(153..277)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(299..644)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(336..356)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
       61 gacggatcct aactcaaaat ccacacatta tacgagccgg aagcataaag tgtaaagcct
      121 ggggtgccta atgcggcccc tcgaggggcc gccatagtga ctggatatgt tgtgttttac
      181 agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat
      241 tttacgtttc tcgttcagct tttttgtaca aacttgttga taactctaga gtcccccgtg
      301 ttctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg
      361 attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg
      421 tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac
      481 cactgtcggc agaggcatct tcaacgatgg cctttccttt atcgcaatga tggcatttgt
      541 aggagccacc ttccttttcc actatcttca caataaagtg acagatagct gggcaatgga
      601 atccgaggag gtttccggat attacccttt gttgaaaagt ctcaattgcc ctttggtctt
      661 ctgagactgt atctttgata tttttggagt agacaagtgt gtcgtgctcc accatgttga
      721 cgaagatttt cttcttgtca ttgagtcgta agagactctg tatgaactgt tcg
//
