LOCUS       sequence-152664-       10809 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Third generation Self-inactivating lentivector expressing NS1 
            (Variant W98G) from Zika virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10809)
  TITLE     Arumugaswami Lab Plasmids
REFERENCE   2  (bases 1 to 10809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLV_Zika_NS1_Flag (Variant: W98G)
FEATURES             Location/Qualifiers
     source          1..10809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..330
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             348..528
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    575..700
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1193..1426
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1611..1655
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1953..2070
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2148..2359
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2372..2640
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      2670..2679
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3747..3770
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3819..4391
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3986..4003)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4213..4232
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4394..4996
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     4893..4913
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5012..5600
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5065..5085)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5483..5494)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6127..6307
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6333..6350)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6339..6563
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6609..7037
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6696..6715)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6906..6927
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7046..7066)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7051..7380
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7231..7366
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7293..7312
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7428..7475
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7494..7868
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7998..8119
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8035..8054)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8089..8108
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8168..8184)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8168..8184)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8181..8203)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8192..8208
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8216..8222,8223..8240,8241..8246))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8261..8282
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8399..8416)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8570..9158)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8650..8669)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9329..10120,10121..10189))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9952..9971
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10190..10294)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10369..10388)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(10560..10809,1..130)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt
      361 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      421 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      481 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      541 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      601 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      661 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      721 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      781 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      841 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      901 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      961 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     1021 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     1081 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1141 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1201 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1261 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1321 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1381 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1441 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1501 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1561 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1621 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1681 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1741 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1801 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1861 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     1921 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     1981 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     2041 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     2101 gtttggttaa ttctagtgga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac
     2161 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag
     2221 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     2281 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     2341 gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg
     2401 ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg
     2461 tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg
     2521 cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct
     2581 gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc
     2641 caagctgtga ccggcgccta ccaccggtcg ccaccatgga tgtggggtgc tcggtggact
     2701 tctcaaagaa ggagacgaga tgcggtacag gggtgttcgt ctataacgac gttgaagcct
     2761 ggagggacag gtacaagtac catcctgact ccccccgtag attggcagca gcagtcaagc
     2821 aagcctggga agatggtatc tgcgggatct cctctgtttc aagaatggaa aacatcatgt
     2881 ggagatcagt agaaggggag ctcaacgcaa tcctggaaga gaatggagtt caactgacgg
     2941 tcgttgtggg atctgtaaaa aaccccatgg ggagaggtcc acagagattg cccgtgcctg
     3001 tgaacgagct gccccacggc tggaaggctt gggggaaatc gtatttcgtc agagcagcaa
     3061 agacaaataa cagctttgtc gtggatggtg acacactgaa ggaatgccca ctcaaacata
     3121 gagcatggaa cagctttctt gtggaggatc atgggttcgg ggtatttcac actagtgtct
     3181 ggctcaaggt tagagaagat tattcattag agtgtgatcc agccgttatt ggaacagctg
     3241 ttaagggaaa ggaggctgta cacagtgatc taggctactg gattgagagt gagaagaatg
     3301 acacatggag gctgaagagg gcccatctga tcgagatgaa aacatgtgaa tggccaaagt
     3361 cccacacatt gtggacagat ggaatagaag agagtgatct gatcataccc aagtctttag
     3421 ctgggccact cagccatcac aataccagag agggctacag gacccaaatg aaagggccat
     3481 ggcacagtga agagcttgaa attcggtttg aggaatgccc aggcactaag gtccacgtgg
     3541 aggaaacatg tggaacaaga ggaccatctc tgagatcaac cactgcaagc ggaagggtga
     3601 tcgaggaatg gtgctgcagg gagtgcacaa tgcccccact gtcgttccgg gctaaagatg
     3661 gctgttggta tggaatggag ataaggccca ggaaagaacc agaaagcaac ttagtaaggt
     3721 caatggtgac tgcatccggg atggccgact acaaagacga cgatgacaag tagcactcga
     3781 ggatatcgac gcgtgaagct tagcggccgc aaattccgcc cctctccctc ccccccccct
     3841 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     3901 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     3961 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     4021 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     4081 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     4141 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     4201 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     4261 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     4321 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     4381 acacgataat accatggcca ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     4441 cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     4501 caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     4561 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     4621 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gctcgcgcat
     4681 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     4741 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     4801 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgctgg
     4861 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     4921 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     4981 caagcccggt gcctgagttc gcgtctggaa caatcaacct ctggattaca aaatttgtga
     5041 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     5101 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     5161 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     5221 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     5281 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     5341 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     5401 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     5461 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     5521 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     5581 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga
     5641 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     5701 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     5761 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     5821 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     5881 cctgattggc agaactacac accagggcca gggatcagat atccactgac ctttggatgg
     5941 tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac
     6001 acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta
     6061 gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac
     6121 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6181 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6241 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6301 tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     6361 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     6421 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     6481 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     6541 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     6601 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     6661 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     6721 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6781 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     6841 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     6901 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     6961 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     7021 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     7081 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     7141 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     7201 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     7261 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     7321 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     7381 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat
     7441 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca
     7501 agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct
     7561 ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc
     7621 gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc
     7681 tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt
     7741 ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg
     7801 ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc
     7861 aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     7921 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     7981 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     8041 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     8101 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     8161 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     8221 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     8281 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     8341 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     8401 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     8461 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     8521 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8581 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8641 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8701 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8761 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8821 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8881 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8941 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     9001 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     9061 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     9121 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     9181 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     9241 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     9301 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     9361 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     9421 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     9481 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     9541 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     9601 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     9661 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9721 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9781 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9841 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9901 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9961 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    10021 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    10081 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    10141 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    10201 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    10261 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    10321 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
    10381 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
    10441 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
    10501 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
    10561 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
    10621 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
    10681 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
    10741 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
    10801 agtgtatca
//
