LOCUS       sequence-152678-        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flp recombinase dependent on GFP (Flp-DOG) for expression in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Tang JC, Drokhlyansky E, Etemad B, Rudolph S, Guo B, Wang S, Ellis 
            EG, Li JZ, Cepko CL
  TITLE     Detection and manipulation of live antigen-expressing cells using 
            conditionally stable nanobodies.
  JOURNAL   Elife. 2016 May 20;5. pii: e15312. doi: 10.7554/eLife.15312.
  PUBMED    27205882
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-05-20"; volume: "5. pii: e15312. doi"; pages: " 
            10.7554/eLife.15312"
COMMENT     Sequence Label: pCAG-Flp-DOG
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      50..59
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggcaaagaat tctgcagtcg acggtaccgc gggcccggga tccaccggtg ccaccatggc
       61 cgacgtgcag ctcgtggaat ccggaggagc actggtccag cctgggggat cactgagact
      121 gtcttgtgtc gcttctggct ttcctgtgaa tcggtactct atgagatggt acaggcaggc
      181 acctggaaaa gagcgagaat gggtcgccgg aatgtcatct gctggcgata ggagttccta
      241 cgtggatagc gtgaaaggac ggttcaccat tagaagggac gatgctagaa acaccgtgta
      301 cctccagatg aactccctga aacctgagga cactgctgtg tactactaca acgtcaatgt
      361 gggctttgaa tactggggac atggcacaca agtgactgtt agttttgcat gcatggctga
      421 cgtccaactt gttgagagcg gtggagcact ggtccagcct gggggatcac tgagactgtc
      481 ttgtgtcgct tctggctttc ctgtgaatcg gtactctatg agatggtaca ggcaggcacc
      541 tggaaaagag cgagaatggg tcgccggaat gtcatctgct ggcgatagga gttcctacgt
      601 ggatagcgtg aaaggacggt tcaccattag aagggacgat gctagaaaca ccgtgtacct
      661 ccagatgaac tccctgaaac ctgaggacac tgctgtgtac tactacaacg tcaatgtggg
      721 ctttgaatac tggggccatg ggacacaggt tactgttagc ttcgcatgca tgagccagtt
      781 cgacatcctg tgcaagaccc cccccaaggt gctggtgcgg cagttcgtgg agagattcga
      841 gaggcccagc ggcgagaaga tcgccagctg tgccgccgag ctgacctacc tgtgctggat
      901 gatcacccac aacggcaccg ccatcaagag ggccaccttc atgagctaca acaccatcat
      961 cagcaacagc ctgagcttcg acatcgtgaa caagagcctg cagttcaagt acaagaccc
//
