LOCUS       sequence-152683-         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flp-dependent Chr2-mCherry encoded in an AAV vector for rAAV 
            production and expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Tang JC, Drokhlyansky E, Etemad B, Rudolph S, Guo B, Wang S, Ellis 
            EG, Li JZ, Cepko CL
  TITLE     Detection and manipulation of live antigen-expressing cells using 
            conditionally stable nanobodies.
  JOURNAL   Elife. 2016 May 20;5. pii: e15312. doi: 10.7554/eLife.15312.
  PUBMED    27205882
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-05-20"; volume: "5. pii: e15312. doi"; pages: " 
            10.7554/eLife.15312"
COMMENT     Sequence Label: pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    61..94
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    105..138
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      169..178
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcaatctttc acaaattttg taatccagag gttgattatc gataagcttg atgatcttcg
       61 gaagttccta ttcttcaaat agtataggaa cttctgcgat tcgggaagtt cctattctct
      121 agaaagtata ggaacttcca aggccggcca ggttggcgcg ccgctagcgc caccatggac
      181 tatggcggcg ctttgtctgc cgtcggacgc gaacttttgt tcgttactaa tcctgtggtg
      241 gtgaacgggt ccgtcctggt ccctgaggat caatgttact gtgccggatg gattgaatct
      301 cgcggcacga acggcgctca gaccgcgtca aatgtcctgc agtggcttgc agcaggattc
      361 agcattttgc tgctgatgtt ctatgcctac caaacctgga aatctacatg cggctgggag
      421 gagatctatg tgtgcgccat tgaaatggtt aaggtgattc tcgagttctt ttttgagttt
      481 aagaatccct ctatgctcta ccttgccaca ggacaccggg tgcagtggct gcgctatgca
      541 gagtggctgc tcacttgtcc tgtcatcctt atccgcctga gcaacctcac cggcctgagc
      601 aacgactaca gcaggagaac catgggactc cttgtctcag acatcgggac tatcgtgtgg
      661 ggggctacca gcgccatggc aaccggctat gttaaagtca tcttcttttg tcttggattg
      721 tgctatggcg cgaacacatt ttttcacgcc gccaaagcat atatcgaggg ttatcatact
      781 gtgccaaagg gtcggtgccg ccaggtcgtg accggcatgg catggctgtt tttcgtgagc
      841 tggggtatgt tcccaattct cttcattttg gggcccgaag gttttggcgt cctgagcgtc
      901 tatggctcca ccgtaggtca cacgattatt gatctgatga gtaaaaattg ttgggggttg
      961 ttgggacact
//
