LOCUS       sequence-152691-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [multiplexing]; p35S:Cas9, nptII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE1
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(52..154)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        594..939
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     882..902
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
       61 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
      121 atggtgaaag ttggaacctc ttacgtgccg atcaacgtct caggtctctg caagaattca
      181 agcttggagg tcaacatggt ggagcacgac actctggtct actccaaaaa tgtcaaagat
      241 acagtctcag aagatcaaag ggctattgag acttttcaac aaaggataat ttcgggaaac
      301 ctcctcggat tccattgccc agctatctgt cacttcatcg aaaggacagt agaaaaggaa
      361 ggtggctcct acaaatgcca tcattgcgat aaaggaaagg ctatcattca agatctctct
      421 gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagag
      481 gttccaacca cgtctacaaa gcaagtggat tgatgtgata acatggtgga gcacgacact
      541 ctggtctact ccaaaaatgt caaagataca gtctcagaag atcaaagggc tattgagact
      601 tttcaacaaa ggataatttc gggaaacctc ctcggattcc attgcccagc tatctgtcac
      661 ttcatcgaaa ggacagtaga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa
      721 ggaaaggcta tcattcaaga tctctctgcc gacagtggtc ccaaagatgg acccccaccc
      781 acgaggagca tcgtggaaaa agaagaggtt ccaaccacgt ctacaaagca agtggattga
      841 tgtgacatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct
      901 tcctctatat aaggaagttc atttcatttg gagaggacac gctcgagtat aagagctcat
      961 ttttaca
//
