LOCUS       sequence-152697-       11604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Third generation Self-inactivating lentivector expressing NS3 from 
            Zika virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11604)
  TITLE     Arumugaswami Lab Plasmids
REFERENCE   2  (bases 1 to 11604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLV_Zika_NS3_Flag
FEATURES             Location/Qualifiers
     source          1..11604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..330
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             348..528
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    575..700
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1193..1426
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1611..1655
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1953..2070
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2148..2359
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2372..2640
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4542..4565
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4614..5186
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4781..4798)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5008..5027
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5189..5791
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5688..5708
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5807..6395
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5860..5880)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6278..6289)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6922..7102
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7128..7145)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7134..7358
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7404..7832
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7491..7510)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7701..7722
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7841..7861)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7846..8175
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8026..8161
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8088..8107
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8223..8270
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8289..8663
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8793..8914
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8830..8849)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8884..8903
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8963..8979)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8963..8979)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8976..8998)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8987..9003
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9011..9017,9018..9035,9036..9041))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9056..9077
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9194..9211)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9365..9953)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9445..9464)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10124..10915,10916..10984))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10747..10766
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10985..11089)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11164..11183)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(11355..11604,1..130)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt
      361 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      421 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      481 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      541 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      601 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      661 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      721 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      781 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      841 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      901 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      961 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     1021 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     1081 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1141 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1201 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1261 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1321 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1381 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1441 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1501 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1561 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1621 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1681 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1741 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1801 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1861 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     1921 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     1981 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     2041 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     2101 gtttggttaa ttctagtgga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac
     2161 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag
     2221 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     2281 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     2341 gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg
     2401 ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg
     2461 tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg
     2521 cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct
     2581 gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc
     2641 caagctgtga ccggcgccta ccaccggtcg ccaccatgag tggtgctcta tgggatgtgc
     2701 ctgctcccaa ggaagtaaaa aagggggaga ccacagatgg agtgtacaga gtaatgactc
     2761 gtagactgct aggttcaaca caagttggag tgggagttat gcaagagggg gtctttcaca
     2821 ctatgtggca cgtcacaaaa ggatccgcgc tgagaagcgg tgaagggaga cttgatccat
     2881 actggggaga tgtcaagcag gatctggtgt catactgtgg tccatggaag ctagatgccg
     2941 cctgggatgg gcacagcgag gtgcagctct tggccgtgcc ccccggagag agagcgagga
     3001 acatccagac tctgcccgga atatttaaga caaaggatgg ggacattgga gcggttgcgc
     3061 tggattaccc agcaggaact tcaggatctc caatcctaga caagtgtggg agagtgatag
     3121 gactttatgg caatggggtc gtgatcaaaa acgggagtta tgttagtgcc atcacccaag
     3181 ggaggaggga ggaagagact cctgttgagt gcttcgagcc ctcgatgctg aagaagaagc
     3241 agctaactgt cttagacttg catcctggag ctgggaaaac caggagagtt cttcctgaaa
     3301 tagtccgtga agccataaaa acaagactcc gtactgtgat cttagctcca accagggttg
     3361 tcgctgctga aatggaggag gcccttagag ggcttccagt gcgttatatg acaacagcag
     3421 tcaatgtcac ccactctgga acagaaatcg tcgacttaat gtgccatgcc accttcactt
     3481 cacgtctact acagccaatc agagtcccca actataatct gtatattatg gatgaggccc
     3541 acttcacaga tccctcaagt atagcagcaa gaggatacat ttcaacaagg gttgagatgg
     3601 gcgaggcggc tgccatcttc atgaccgcca cgccaccagg aacccgtgac gcatttccgg
     3661 actccaactc accaattatg gacaccgaag tggaagtccc agagagagcc tggagctcag
     3721 gctttgattg ggtgacggat cattctggaa aaacagtttg gtttgttcca agcgtgagga
     3781 acggcaatga gatcgcagct tgtctgacaa aggctggaaa acgggtcata cagctcagca
     3841 gaaagacttt tgagacagag ttccagaaaa caaaacatca agagtgggac tttgtcgtga
     3901 caactgacat ttcagagatg ggcgccaact ttaaagctga ccgtgtcata gattccagga
     3961 gatgcctaaa gccggtcata cttgatggcg agagagtcat tctggctgga cccatgcctg
     4021 tcacacatgc cagcgctgcc cagaggaggg ggcgcatagg caggaatccc aacaaacctg
     4081 gagatgagta tctgtatgga ggtgggtgcg cagagactga cgaagaccat gcacactggc
     4141 ttgaagcaag aatgctcctt gacaatattt acctccaaga tggcctcata gcctcgctct
     4201 atcgacctga ggccgacaaa gtagcagcca ttgagggaga gttcaagctt aggacggagc
     4261 aaaggaagac ctttgtggaa ctcatgaaaa gaggagatct tcctgtttgg ctggcctatc
     4321 aggttgcatc tgccggaata acctacacag atagaagatg gtgctttgat ggcacgacca
     4381 acaacaccat aatggaagac agtgtgccgg cagaggtgtg gaccagacac ggagagaaaa
     4441 gagtgctcaa accgaggtgg atggacgcca gagtttgttc agatcatgcg gccctgaagt
     4501 cattcaagga gtttgccgct gggaaaagat ccgggatggc cgactacaaa gacgacgatg
     4561 acaagtagca ctcgaggata tcgacgcgtg aagcttagcg gccgcaaatt ccgcccctct
     4621 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     4681 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     4741 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     4801 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     4861 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     4921 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     4981 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     5041 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     5101 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     5161 gttttccttt gaaaaacacg ataataccat ggccaccgag tacaagccca cggtgcgcct
     5221 cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta
     5281 ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca
     5341 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     5401 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     5461 gatcggctcg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     5521 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     5581 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     5641 ggccgagcgc gctggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     5701 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     5761 ctggtgcatg acccgcaagc ccggtgcctg agttcgcgtc tggaacaatc aacctctgga
     5821 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     5881 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     5941 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     6001 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     6061 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     6121 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     6181 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     6241 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     6301 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     6361 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac
     6421 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     6481 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     6541 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     6601 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     6661 acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca
     6721 ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc
     6781 aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     6841 gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca catggcccga
     6901 gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     6961 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7021 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7081 tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     7141 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     7201 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     7261 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     7321 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     7381 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     7441 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     7501 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     7561 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     7621 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     7681 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     7741 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     7801 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     7861 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7921 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7981 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     8041 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     8101 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     8161 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     8221 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     8281 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
     8341 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
     8401 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
     8461 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
     8521 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
     8581 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
     8641 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct
     8701 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     8761 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     8821 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     8881 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     8941 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     9001 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     9061 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     9121 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     9181 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     9241 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     9301 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     9361 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     9421 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     9481 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     9541 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     9601 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     9661 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     9721 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     9781 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     9841 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     9901 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     9961 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    10021 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
    10081 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    10141 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    10201 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    10261 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    10321 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    10381 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    10441 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    10501 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    10561 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    10621 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    10681 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    10741 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    10801 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    10861 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    10921 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    10981 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    11041 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    11101 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
    11161 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
    11221 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
    11281 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
    11341 cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
    11401 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
    11461 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
    11521 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
    11581 cttggcagta catcaagtgt atca
//
