LOCUS       sequence-152704-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA shuttle vector; module 4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE10
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(49..151)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(305..322)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(305..321)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(314..336)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(530..549)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     649..671
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(709..727)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        795..899
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
       61 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
      121 gtgaaagttg gaacctctta cgtgccgatc aacgtctcag aagacttcaa tcactacttc
      181 gactctagct gtatataaac tcagcttcgt tttcttatct aagcgatgtg ggacttttga
      241 agattgtttt caacttacca ttgagaccat ctagatgcat tcgcgaggta ccgagctcga
      301 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
      361 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      421 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
      481 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
      541 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
      601 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
      661 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
      721 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
      781 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      841 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      901 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
      961 tttttgctca ccca
//
