LOCUS       sequence-152738-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BxbI attL-attR. GFP expression will stop if sites recombine..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Roquet N, Soleimany AP, Ferris AC, Aaronson S, Lu TK
  TITLE     Synthetic recombinase-based state machines in living cells.
  JOURNAL   Science. 2016 Jul 22;353(6297):aad8559. doi: 
            10.1126/science.aad8559. Epub 2016 Jul 21.
  PUBMED    27463678
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-07-22"; volume: "353(6297):aad8559. doi"; pages: " 
            10.1126/science.aad8559. Epub 2016 Jul 21"
COMMENT     Sequence Label: pNR279
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..23
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_RNA        complement(56..105)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     primer_bind     complement(339..357)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcatggtac ctttctcctc tttaatgaat tcttaaacaa aattatttgt agaggctgtt
       61 tcgtcctcac ggactcatca gaccggaaag cacatccggt gacagctgtg cacgtcgggg
      121 tttgtaccgt acaccactga gaccgccgtc gtcgacaagc cggccgttaa ttaaaaagtt
      181 aaacaaaatt atttgtagag ggaaaccgtt gtggtctccc tgaatatatt atacgagcct
      241 tatgcatgcc cgtaaagtta tccagcaacc actcatagac ctagggcagc agatagggac
      301 gacgtggtgt tagctgtgct cgaggtgaag acgaaagggc ctcgtgatac gcctattttt
      361 ataggttaat gtcatgataa taatggtttc ttagacgtcg agtcttacgc cccgccctgc
      421 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
      481 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
      541 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
      601 ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta
      661 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
      721 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
      781 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
      841 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
      901 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
      961 tggttatagg tacattgagc aactgactga
//
