LOCUS       sequence-152741-         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BxbI attB-attP. GFP will express if sites recombine. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Roquet N, Soleimany AP, Ferris AC, Aaronson S, Lu TK
  TITLE     Synthetic recombinase-based state machines in living cells.
  JOURNAL   Science. 2016 Jul 22;353(6297):aad8559. doi: 
            10.1126/science.aad8559. Epub 2016 Jul 21.
  PUBMED    27463678
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-07-22"; volume: "353(6297):aad8559. doi"; pages: " 
            10.1126/science.aad8559. Epub 2016 Jul 21"
COMMENT     Sequence Label: pNR230
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             11..22
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_RNA        complement(55..104)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     protein_bind    113..170
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attP site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
ORIGIN
        1 tcatggtacc tttctcctct ttaatgaatt cttaaacaaa attatttgta gaggctgttt
       61 cgtcctcacg gactcatcag accggaaagc acatccggtg acagctgtgc acgtcggggt
      121 ttgtaccgta caccactgag accgcggtgg ttgaccagac aaaccacgac gctagcccgc
      181 cacctaagtt atgagtcagg aaaaaaggcg acagagtaat ctgtcgcctt ttttctttgc
      241 ttgctttgta atcactcgat gaccaaaagt aaaaacccgc cgaagcgggt ttttacgtaa
      301 aacaggtgaa actgacgcta gccggtcaca tgtcaatact tgcccttgac aggcattgat
      361 ggaatcgtag tctcacgctg atagtctgat cgacaataca agtgggaccg tggtcccaga
      421 ccgataatca gaccgacaac acgagtggga tcgtggtccc agactaataa tcagaccgac
      481 gatacgagtg ggaccgtggt cccagactaa taatcagacc gacgatacga gtgggaccgt
      541 ggttccagac taataatcag accgacgata cgagtgggac cgtggtccca gactaataat
      601 cagaccgacg atacgagtgg gaccatggtc ccagactaat aatcagaccg acgatacgag
      661 tgggaccgtg gtcccagtct gattatcaga ccgacgatac gagtgggacc gtggtcccag
      721 actaataatc agaccgacga tacgagtggg accgtggtcc cagtctgatt atcagaccga
      781 cgatacaagt ggaacagtgg gcccagagag aatattcagg ccagttatgc tttctggcct
      841 gtaacaaagg acattaagta aagacagata aacgtagact aaaacgtggt cgcatcaggg
      901 tgctggct
//
