LOCUS       sequence-152742-         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Output plasmid: contains 5-state register that regulates expression 
            of GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Roquet N, Soleimany AP, Ferris AC, Aaronson S, Lu TK
  TITLE     Synthetic recombinase-based state machines in living cells.
  JOURNAL   Science. 2016 Jul 22;353(6297):aad8559. doi: 
            10.1126/science.aad8559. Epub 2016 Jul 21.
  PUBMED    27463678
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-07-22"; volume: "353(6297):aad8559. doi"; pages: " 
            10.1126/science.aad8559. Epub 2016 Jul 21"
COMMENT     Sequence Label: pNR165
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             11..19
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_RNA        complement(62..111)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     protein_bind    419..467
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     protein_bind    765..822
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attP site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
ORIGIN
        1 ctcattagtg acctccttaa tataggtcgg cttcgacctt aaacaaaatt atttgtagag
       61 gctgtttcgt cctcacggac tcatcagacc ggaaagcaca tccggtgaca gctgtgccat
      121 ccctcgtcct cacgagtgcg gtattcctct gggcatgcca acacaattaa catctcaatc
      181 aaggtaaatg ctttttgctt tttttgccgt ggtgcggatc tagctacctc ggcgttaatc
      241 cttgttcact atatgcaacc attatcaccg ccagaggtaa aatagtcaaa cgcacggtgt
      301 tagccgcact tgagtccacg gctgatgcca acacaattaa catcagaatc aaggtaaatg
      361 ctttttgctt tttttgcggg ctcactaggg aatggcgtcg tccgtgacat tctgtgcgcg
      421 gccggcttgt cgacgacggc ggtctccgtc gtcaggatca tccgggccag cggctgtcta
      481 tgccagaagt cggtcaggtc ggagtcctag aaaggagttt tttagttacc ttaattctaa
      541 taaacgaaat aaaaactcgc cgttcgcgcg acatgacatg cgccgccgat tgattcagaa
      601 agttaaacaa aattatttgt agagggaaac cgttgtggtc tccctgaata tattatacga
      661 gccttatgca tgcccgtaaa gttatccagc aaccactcat agacctaggg cagcagatag
      721 ggacgacgtg gtgttagctg tgccgacaca atctccctgc tcaggtcgtg gtttgtctgg
      781 tcaaccaccg cggtctcagt ggtgtacggt acaaaccccg acgcaatagc gccaaccatg
      841 caccatagcc agcctgacag tagccaaagg agttttttag ttaccttaat tgaaataaac
      901 gaaata
//
