LOCUS       sequence-152748-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression of EPN-01* in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pET29b-EPN-01*
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             664..693
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atgggtgcgc gtgcgtctgg ttctaaatct ggctccggct ctgactctgg tagcaaaatg
       61 gaagagctgt tcaagaaaca caagatcgtt gccgtgctgc gtgccaatag tgtggaagaa
      121 gcgaaaaaga aagcgctggc ggttttcctg ggcggcgttc atctgattga aattaccttt
      181 accgtgccgg atgcggatac cgtgattaag gaactgagct ttctgaagga aatgggcgcg
      241 attattggtg cgggcaccgt gaccagcgtg gagcagtgcc gtaaagcggt ggaaagtggc
      301 gccgaattca ttgtgagtcc gcacctggac gaggaaatta gccaattttg caaggagaag
      361 ggtgtgttct atatgccagg cgttatgacc ccgaccgaac tggtgaaagc catgaaactg
      421 ggccatacca tcttaaaact gtttccgggt gaggtggtgg gtccgcagtt tgttaaagcg
      481 atgaaaggtc cgtttccgaa tgtgaaattt gtgccaaccg gcggtgttaa tctggacaat
      541 gtgtgcgaat ggttcaaagc gggcgtgctg gccgtgggcg tgggcagcgc gttagtgaaa
      601 ggcaccccgg tggaagtggc ggaaaaggcc aaggcgttcg ttgagaagat tcgtggctgc
      661 accgaacaga aactgattag cgaagaagat ctgcagtctc gtccggaacc gaccgcaccg
      721 ccggaagaat ctttccgttc tggtgttgaa accaccacgc caccgcaaaa acaggagccg
      781 atcgacaaag aactgtaccc gctgacctct ctgcgttctc tgttcggtaa cgacccgtct
      841 tctcagtga
//
