LOCUS       sequence-152758-        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) 
            linked to IRES-mCherry under the control of TetOp and a minimal CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   McDonald JI, Celik H, Rois LE, Fishberger G, Fowler T, Rees R, 
            Kramer A, Martens A, Edwards JR, Challen GA
  TITLE     Reprogrammable CRISPR/Cas9-based system for inducing site-specific 
            DNA methylation.
  JOURNAL   Biol Open. 2016 May 11. pii: bio.019067. doi: 10.1242/bio.019067.
  PUBMED    27170255
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol Open. 
            2016 May 11. pii: bio.019067. doi: 10.1242/bio.019067."
COMMENT     Sequence Label: TetO-dCas9-D3A
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      79..88
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccc cgaattcgcc
       61 atacattcta gaggatccgc caccatggac aagaagtact ccattgggct cgctatcggc
      121 acaaacagcg tcggctgggc cgtcattacg gacgagtaca aggtgccgag caaaaaattc
      181 aaagttctgg gcaataccga tcgccacagc ataaagaaga acctcattgg cgccctcctg
      241 ttcgactccg gggagacggc cgaagccacg cggctcaaaa gaacagcacg gcgcagatat
      301 acccgcagaa agaatcggat ctgctacctg caggagatct ttagtaatga gatagctaag
      361 gtggatgact ctttcttcca taggctggag gagtcctttt tggtggagga ggataaaaag
      421 cacgagcgcc acccaatctt tggcaatatc gtggacgagg tggcgtacca tgaaaagtac
      481 ccaaccatat atcatctgag gaagaagctt gtagacagta ctgataaggc tgacttgcgg
      541 ttgatctatc tcgcgctggc gcatatgatc aaatttcggg gacacttcct catcgagggg
      601 gacctgaacc cagacaacag cgatgtcgac aaactcttta tccaactggt tcagacttac
      661 aatcagcttt tcgaagagaa cccgatcaac gcatccggag ttgacgccaa agcaatcctg
      721 agcgctaggc tgtccaaatc ccggcggctc gaaaacctca tcgcacagct ccctggggag
      781 aagaagaacg gcctgtttgg taatcttatc gccctgtcac tcgggctgac ccccaacttt
      841 aaatctaact tcgacctggc cgaagatgcc aagcttcaac tgagcaaaga cacctacgat
      901 gatgatctcg acaatctgct ggcccagatc ggcgaccagt acgcagacct ttttttggcg
      961 gcaaagaacc tgtcagacgc cattctgctg agtgatattc tgcgagtgaa cacggagatc
     1021 accaaagct
//
