LOCUS       sequence-152759-        7837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one doxycycline inducible lentiviral vector for expression of
            one or two genes using the P2A self-cleaving peptide..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Barger CJ, Branick C, Chee L, Karpf AR
  TITLE     Pan-Cancer Analyses Reveal Genomic Features of FOXM1 Overexpression 
            in Cancer.
  JOURNAL   Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 
            10.3390/cancers11020251.
  PUBMED    30795624
REFERENCE   2  (bases 1 to 7837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancers 
            (Basel)."; date: "2019-02-21"; volume: "11(2). pii: cancers11020251.
            doi"; pages: " 10.3390/cancers11020251"
COMMENT     Sequence Label: pCW57-MCS1-P2A-MCS2 (GFP)
FEATURES             Location/Qualifiers
     source          1..7837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             567..623
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        651..1161
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1080..1098
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      1179..1188
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1185..1880
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
     CDS             1896..1949
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(1950..2567,2568..2573,2574..2609,2610..2612,2613..2648,
                     2649..2651,2652..2687,2688..2696)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(2736..2756)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3154..3165)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3370..3603
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3675..3796
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3712..3731)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3766..3785
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3836..3971
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        3971..4042
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4043..4111,4112..4903)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4261..4280)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5074..5662
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5563..5582
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5816..5833
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5950..5971
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5986..5991,5992..6009,6010..6016)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6024..6040
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6029..6051
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6048..6064
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6048..6064
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6083..6103
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6085..6103
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6131..6357
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6358..6538
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6585..6710
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7203..7436
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7621..7665
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcgaattcgt cgacaccggt tccggagcca cgaacttctc tctgttaaag caagcaggag
      601 acgtggaaga aaaccccggt cctaggctgc agacgcgttg tacaggatcc ggggttgggg
      661 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
      721 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
      781 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
      841 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
      901 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
      961 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
     1021 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
     1081 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
     1141 atcaccgacc tctctcccca gcaattcacc cgcgcgcggc caccatggag agcgacgaga
     1201 gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt
     1261 tcgagctggt gggcggcgga gagggcaccc ccgagcaggg ccgcatgacc aacaagatga
     1321 agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg atgggctacg
     1381 gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg cacgccatca
     1441 acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc gtgctgcacg
     1501 tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag gtgatgggca
     1561 ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc aacgccaccg
     1621 tggagcacct gcaccccatg ggcgataacg atctggatgg cagcttcacc cgcaccttca
     1681 gcctgcgcga cggcggctac tacagctccg tggtggacag ccacatgcac ttcaagagcg
     1741 ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc cgcgtggagg
     1801 aggatcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc aagaccccgg
     1861 atgcagatgc cggtgaagaa ttcgaaggaa gcggagaagg tagaggttct ctcctcactt
     1921 gtggtgatgt tgaagaaaac cctggtccaa tgtctagact ggacaagagc aaagtcataa
     1981 acggagctct ggaattactc aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg
     2041 ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc
     2101 tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg
     2161 aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcataccgc tgtgctctcc
     2221 tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg
     2281 aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac
     2341 tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc
     2401 atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga
     2461 gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg
     2521 aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccgaccgacg
     2581 cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg
     2641 atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtaactaa
     2701 gtaaggatcg atccaagata tcgtattctt aactatgttg ctccttttac gctatgtgga
     2761 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     2821 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     2881 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     2941 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     3001 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     3061 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     3121 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     3181 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     3241 agtcggatct ccctttgggc cgcctccccg cctgtttcgc ctcggcgtcc ggactagagg
     3301 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     3361 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     3421 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     3481 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     3541 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     3601 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     3661 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     3721 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     3781 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     3841 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     3901 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     3961 ttttggaggc ctttcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     4021 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     4081 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     4141 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     4201 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     4261 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     4321 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     4381 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     4441 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     4501 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     4561 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     4621 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     4681 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     4741 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     4801 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     4861 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     4921 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     4981 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     5041 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5101 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5161 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5221 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5281 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5341 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5401 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5461 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5521 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5581 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5641 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5701 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     5761 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     5821 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     5881 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     5941 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     6001 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     6061 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
     6121 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
     6181 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
     6241 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
     6301 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
     6361 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     6421 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     6481 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     6541 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     6601 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     6661 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     6721 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     6781 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     6841 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     6901 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     6961 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     7021 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     7081 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     7141 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     7201 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     7261 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     7321 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     7381 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     7441 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     7501 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     7561 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     7621 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     7681 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     7741 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     7801 ttatcgtttc agacccacct cccaaccccg aggggac
//
