LOCUS       sequence-152764-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) 
            linked to IRES-mCherry under the control of TetOp and a minimal CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   McDonald JI, Celik H, Rois LE, Fishberger G, Fowler T, Rees R, 
            Kramer A, Martens A, Edwards JR, Challen GA
  TITLE     Reprogrammable CRISPR/Cas9-based system for inducing site-specific 
            DNA methylation.
  JOURNAL   Biol Open. 2016 May 11. pii: bio.019067. doi: 10.1242/bio.019067.
  PUBMED    27170255
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol Open. 
            2016 May 11. pii: bio.019067. doi: 10.1242/bio.019067."
COMMENT     Sequence Label: TetO-dCas9-D3A
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agaagacagg gccaggtttc cgggccctca cattgccaaa agacggcaat atggtggaaa
       61 ataacatata gacaagcgca caccggcctt attccaagcg gcttcggcca gtaacgttag
      121 gggggggggt cgagtaccgg tttacacaca cgcaaaatac tccttcagcg gagcgaagag
      181 gtggcggatg actggcacgc tccatgaccg gcccagcagt ctctgcctcg ccaagcggct
      241 catgttggag acgtcagtat agtggactgg gaaaccaaat accctttcca tttcagtgca
      301 ccataagatg tcctctttct cattcatgaa gacaggaaaa tgctggtctt tgccctgctt
      361 tatggagttt gacctcgtag taatggtcct cactttgctg aacttggcta tcctgccatg
      421 ctccagacac tcctgcagct ccagcttatc attcacagtg gatgccaacg gcctgttcat
      481 accgggaagg ttaccccaga agtagcgggc cctgtgtgca gctgacactt ctttggcatc
      541 aatcatcaca gggttggact cgagaaatcg cgagatgtcc ctcttgtcac taacgcccat
      601 ggccaccaca ttctcaaaga gccagaagaa ggggcgatca tctccctcct tgggccgcgc
      661 atcatgcagg aggcggtaga actcaaagaa gagccggcca gtgccctcgt agaggccctt
      721 gcgagcaggg ttgacgatgg agaggtcatt gcagggactg cccccaatca ccagatcgaa
      781 tgggccccac tcctggatat gcttctgtgt gacgctgcgg acgtccccga cgtacatgat
      841 cttcccctgg tgccgcacca tgcccaccgt gatggagtcc tcacacacct ccgaggcaat
      901 gtagcggtcc acctgaatgc ccaagtcctt cagcaccagg agccctgtag cgatt
//
