LOCUS       sequence-152774-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one doxycycline inducible lentiviral vector for expression of
            one or two genes using the P2A self-cleaving peptide..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Barger CJ, Branick C, Chee L, Karpf AR
  TITLE     Pan-Cancer Analyses Reveal Genomic Features of FOXM1 Overexpression 
            in Cancer.
  JOURNAL   Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 
            10.3390/cancers11020251.
  PUBMED    30795624
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancers 
            (Basel)."; date: "2019-02-21"; volume: "11(2). pii: cancers11020251.
            doi"; pages: " 10.3390/cancers11020251"
COMMENT     Sequence Label: pCW57-MCS1-P2A-MCS2 (Blast)
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             105..500
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             516..569
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gktssattct gcagctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac
       61 cgacctctct ccccagcaat tcacccgcgc gcgggaacta aaccatggcc aagcctttgt
      121 ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc atccccatct
      181 ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg
      241 tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg
      301 ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca
      361 tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag
      421 ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct
      481 ctggttatgt gtgggagggc ttcgaaggaa gcggagaagg tagaggttct ctcctcactt
      541 gtggtgatgt tgaagaaaac cctggtccaa tgtctagact ggacaagagc aaagtcataa
      601 acggagctct ggaattactc aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg
      661 ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc
      721 tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg
      781 aaaggcgagt catggcaaga ctttctgcgg aacaacgcca agtcataccg ctgtgctctc
      841 tctcamatcg cgacgggggc taagtgcatc tcggcacccg cccaacagag aaacagtacg
      901 aaacctggaa attcagctcg cgtttctgtg tcagcaagct tytccctgga raacgcactg
      961 tacgctctkg tcgccgtkgg tcactttaca ctgtgggcts gtscgtawtt gggarggggc
//
