LOCUS       sequence-152788-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid which encodes kanamycin resistance gene for 
            positive selection and toxin gene (tse2) under the control of 
            rhamnose induceable promoter (PrhaB) for negative selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Khetrapal V, Mehershahi K, Rafee S, Chen S, Lim CL, Chen SL
  TITLE     A set of powerful negative selection systems for unmodified 
            Enterobacteriaceae.
  JOURNAL   Nucleic Acids Res. 2015 Jul 27;43(13):e83. doi: 10.1093/nar/gkv248. 
            Epub 2015 Mar 23.
  PUBMED    25800749
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2015-07-27"; volume: "43(13):e83. doi"; pages: "
            10.1093/nar/gkv248. Epub 2015 Mar 23"
COMMENT     Sequence Label: pSLC-246
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      599..845
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
ORIGIN
        1 ggcgcttttt agactggtcg taatgaaatt cagcaggatc accatatgag gaggtaaaat
       61 agatgtccta cgactacgag aaaaccagcc tcaccctcta ccgggcggta ttcaaggcca
      121 actacgacgg cgacgtcggt cgctacctgc atcccgacaa ggaactcgcc gaggctgcgg
      181 aagtcgcccc gctgctgcat ccgaccttcg acagccccaa cacccctggc gtccccgccc
      241 gcgcgccgga catcgtcgcc ggccgcgacg gcctctacgc cccggacacc ggcggcacct
      301 cggtgttcga ccgcgccggc gtgctgcgcc gcgccgacgg cgacttcgtg atacccgacg
      361 gcaccgacat cccgccggac cttaaggtga agcaggacag ctacaacaag cgcctgcaag
      421 ccacccacta caccatcatg ccggccaagc cgatgtaccg ggaggtcctc atgggccaac
      481 tggacaactt cgtgcgcaac gccatccgcc gccaatggga aaaagcccgc gggctctagg
      541 gatccgcgaa ttctcatgtt tgacagctta tcactgatca gtgaattaat ggcgatgacg
      601 catcctcacg ataatatccg ggtaggcgca atcactttcg tctctactcc gttacaaagc
      661 gaggctgggt atttcccggc ctttctgtta tccgaaatcc actgaaagca cagcggctgg
      721 ctgaggagat aaataataaa cgaggggctg tatgcacaaa gcatcttctg ttgagttaag
      781 aacgagtatc gagatggcac atagccttgc tcaaattgga atcaggtttg tgccaatacc
      841 agtagaaaca gacgaagaag ctagctttgc actggatggg ccgcggagga tcgcttttga
      901 agctggggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg
      961 cgtcccggaa aacgattccg aagcccaacc ttt
//
