LOCUS       sequence-152789-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid which encodes kanamycin resistance gene for 
            positive selection and toxin gene (yhaV) under the control of 
            rhamnose induceable promoter (PrhaB) for negative selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Khetrapal V, Mehershahi K, Rafee S, Chen S, Lim CL, Chen SL
  TITLE     A set of powerful negative selection systems for unmodified 
            Enterobacteriaceae.
  JOURNAL   Nucleic Acids Res. 2015 Jul 27;43(13):e83. doi: 10.1093/nar/gkv248. 
            Epub 2015 Mar 23.
  PUBMED    25800749
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2015-07-27"; volume: "43(13):e83. doi"; pages: "
            10.1093/nar/gkv248. Epub 2015 Mar 23"
COMMENT     Sequence Label: pSLC-239
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      582..828
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     protein_bind    complement(881..914)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gtcgcgaatt caggcgcttt ttagactggt cgtaatgaaa ttcagcagga tcaccatatg
       61 gattttccac aaagggttaa tggttgggcg ctatatgctc atccctgttt tcaggaaacc
      121 tacgacgctt tagttgccga agtcgagaca ttaaagggaa aagatcctga aaattatcag
      181 agaaaagccg ccacaaagtt attggcggta gtccataaag tgattgagga gcatatcacg
      241 gtcaatccat catcaccggc attccgtcat ggcaagtcgt taggctctgg gaaaaataaa
      301 gactggtcac gggtaaaatt tggtgctggt cgttatcgtc tcttctttcg ttatagtgaa
      361 aaagagaaag tcatcattct gggatggatg aacgatgaaa acactctgcg cacctacggt
      421 aaaaaaacag atgcctatac cgtattcagc aaaatgttaa aaagaggaca tcctcctgcc
      481 gactgggaaa ccctcacccg agaaacagaa gaaacccatt gaggatccgc gaattctcat
      541 gtttgacagc ttatcactga tcagtgaatt aatggcgatg acgcatcctc acgataatat
      601 ccgggtaggc gcaatcactt tcgtctctac tccgttacaa agcgaggctg ggtatttccc
      661 ggcctttctg ttatccgaaa tccactgaaa gcacagcggc tggctgagga gataaataat
      721 aaacgagggg ctgtatgcac aaagcatctt ctgttgagtt aagaacgagt atcgagatgg
      781 cacatagcct tgctcaaatt ggaatcaggt ttgtgccaat accagtagaa acagacgaag
      841 aagctagctt tgcactggat ggggacgtca ggatcgctct gaagttccta tactttctag
      901 agaataggaa cttcggaata ggaactaagg aggatattca tatggaccat ggctaattcc
      961 catgtcagcc gttaagtgtt cctgtgtcac t
//
