LOCUS       sequence-152794-         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid which encodes chloramphenicol resistance gene for 
            positive selection and a toxin gene (relE) under the control of 
            rhamnose induceable promoter (PrhaB) for negative selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Khetrapal V, Mehershahi K, Rafee S, Chen S, Lim CL, Chen SL
  TITLE     A set of powerful negative selection systems for unmodified 
            Enterobacteriaceae.
  JOURNAL   Nucleic Acids Res. 2015 Jul 27;43(13):e83. doi: 10.1093/nar/gkv248. 
            Epub 2015 Mar 23.
  PUBMED    25800749
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2015-07-27"; volume: "43(13):e83. doi"; pages: "
            10.1093/nar/gkv248. Epub 2015 Mar 23"
COMMENT     Sequence Label: pSLC-242
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      415..661
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     protein_bind    complement(714..747)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 taacgagaag gtcgcgaatt caggcgcttt ttagactggt cgtaatgaaa ttcagcagga
       61 tcaccatatg gcgtattttc tggattttga cgagcgggca ctaaaggaat ggcgaaagct
      121 gggctcgacg gtacgtgaac agttgaaaaa gaagctggtt gaagtacttg agtcaccccg
      181 gattgaagca aacaagctcc gtggtatgcc tgattgttac aagattaagc tccggtcttc
      241 aggctatcgc cttgtatacc aggttataga cgagaaagtt gtcgttttcg tgatttctgt
      301 tgggaaaaga gaacgctcgg aagtatatag cgaggcggtc aaacgcattc tctgaggatc
      361 cgcgaattct catgtttgac agcttatcac tgatcagtga attaatggcg atgacgcatc
      421 ctcacgataa tatccgggta ggcgcaatca ctttcgtctc tactccgtta caaagcgagg
      481 ctgggtattt cccggccttt ctgttatccg aaatccactg aaagcacagc ggctggctga
      541 ggagataaat aataaacgag gggctgtatg cacaaagcat cttctgttga gttaagaacg
      601 agtatcgaga tggcacatag ccttgctcaa attggaatca ggtttgtgcc aataccagta
      661 gaaacagacg aagaagctag ctttgcactg gatggggacg tcaggatcgc tctgaagttc
      721 ctatactttc tagagaatag gaacttcgga ataggaacta aggaggatat tcatatggac
      781 catggctaat tcccatgtca gccgttaagt gttcctgtgt cactcaaaat tgctttgaga
      841 ggctctaagg gcttctcagt gcgttacatc cctggcttgt tgtccacaac cgttaaacct
      901 taaaagcttt aaaagcctta tatattcttt ttttt
//
