LOCUS       sequence-152814-         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tricistronic expression of TRPV4, ferritin, and mCherry in mammalian
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Wheeler MA, Smith CJ, Ottolini M, Barker BS, Purohit AM, Grippo RM, 
            Gaykema RP, Spano AJ, Beenhakker MP, Kucenas S, Patel MK, Deppmann 
            CD, Guler AD
  TITLE     Genetically targeted magnetic control of the nervous system.
  JOURNAL   Nat Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265.
  PUBMED    26950006
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265."
COMMENT     Sequence Label: pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             394..417
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             433..489
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             523..546
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atcctggaca tcgagcgctc cttccctgtg ttcctgagga aggccttccg ctccggagag
       61 atggtgacag tgggcaagag ctcggatggc actccagacc gcaggtggtg cttcagggtg
      121 gacgaggtga actggtctca ctggaaccag aacctgggca tcattaacga ggaccccggc
      181 aagagcgaga tctaccagta ctatggcttc tcccatacca tggggcgcct ccgcagggat
      241 cgctggtcct cagtggtgcc ccgcgtggtg gagctgaaca agaactcagg cacagatgaa
      301 gtggtggtcc ccctggataa cctagggaac cccaactgtg acggccacca gcaaggttat
      361 gctcccaagt ggagggcgga ggacgcacca ctggattaca aggacgatga cgataaggga
      421 tccggcagcg gcgccaccaa cttcagcctg ctgaagcagg ccggcgacgt ggaggagaac
      481 cccggccccc tcgagtccgg agcacggggc gggggcggtt ctgattataa ggacgacgac
      541 gacaaaggag gaggcggctc ctctcgggtt atgggctccc agattcgtca gaattattcc
      601 accgacgtgg aggcagccgt caacagcctg gtcaatttgt acctgcaggc ctcctacacc
      661 tacctctctc tgggcttcta tttcgaccgc gatgatgtgg ctctggaagg cgtgagccac
      721 ttcttccgcg aattggccga ggagaagcgc gagggctacg agcgtctcct gaagatgcaa
      781 aaccagcgtg gcggccgcgc tctcttccag gacatcaaga agccagctga agatgagtgg
      841 ggtaaaaccc cagacgccat gaaagctgcc atggccctgg agaaaaagct gaaccaggcc
      901 c
//
