LOCUS       sequence-152822-         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SIRT3 via retroviral method.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Jing E, O'Neill BT, Rardin MJ, Kleinridders A, Ilkeyeva OR, Ussar S,
            Bain JR, Lee KY, Verdin EM, Newgard CB, Gibson BW, Kahn CR
  TITLE     Sirt3 regulates metabolic flexibility of skeletal muscle through 
            reversible enzymatic deacetylation.
  JOURNAL   Diabetes. 2013 Oct;62(10):3404-17. doi: 10.2337/db12-1650. Epub 2013
            Jul 8.
  PUBMED    23835326
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.2337/db12-1650";
            journalName: "Diabetes"; date: "2013-10"; volume: "62"; issue: "10";
            pages: "3404-17"
COMMENT     Sequence Label: pBabe-SIRT3
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             846..926
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 cagtgtggtg gtacgtagga attctagctg agctgcttcg gaccagagcc tgcagtaggg
       61 tggtggtcat ggtgggggcc ggcatcagca cacccagtgg catcccggac ttcagatccc
      121 cagggagcgg cctctacagc aaccttcagc agtatgacat cccgtaccct gaagccatct
      181 ttgaacttgg ctttttcttt cacaacccca agcccttttt catgttggcc aaggagctgt
      241 accctgggca ctacaggccc aatgtcactc actacttcct gaggctcctc cacgacaagg
      301 agctgcttct gcggctctat acacagaaca tcgacgggct tgagagagca tctgggatcc
      361 ctgcctcaaa gctggttgaa gcccacggga cctttgtaac agctacatgc acggtctgtc
      421 gaaggtcctt cccaggggaa gacatatggg ctgatgtgat ggcggacagg gtgccccgct
      481 gccctgtctg tactggcgtt gtgaaacccg acattgtgtt ctttggggag cagctgcctg
      541 caaggttcct actccatatg gctgacttcg ctttggcaga tctgctactc attcttggga
      601 cctccctgga ggtggagcct tttgccagct tgtccgaagc agtacagaaa tcagtgcccc
      661 gactgctcat caatcgagac ttggtggggc cgttcgttct gagtcctcga aggaaagatg
      721 tggtccagct aggggatgta gttcatggtg tggaaaggct ggtggacctc ccggggtgga
      781 cacaagaact gctggatctt atgcagcggg aacgtggcaa gctggatgga caggacagac
      841 tcgagtaccc atatgacgtt ccagactacg cgtatccgta cgacgttccg gattacgctt
      901 acccttacga tgtacctgac tacgcttaag tcgaccctgt ggaatgtgtg tcagttaagg
      961 tgtgg
//
